biostudies-literatureEilts FHeinrich Boell FoundationHeinrich Böll FoundationGerman Federal Ministry of Economic Affairs and EnergyGerman Federal Ministry for Economic AffairsUniversity of Applied Sciences MittelhessenEuropean Regional Development Fund1070https://www.ebi.ac.uk/biostudies/studies/S-EPMC9696199biostudies-literatureUnknown12(11)Steric exclusion chromatography (SXC) is a promising purification method for biological macromolecules such as the Orf virus (ORFV) vector. The method's principle is closely related to conventional polyethylene glycol (PEG) precipitation, repeatedly implementing membranes as porous chromatographic media. In the past decade, several purification tasks with SXC showed exceptionally high yields and a high impurity removal. However, the effect of varying process parameters, on the precipitation success and its limitations to SXC, is not yet well understood. For this reason, the precipitation behavior and SXC adaptation for ORFV were investigated for the PEG/ORFV contact time, the membranes pore size, and the type and concentration of ions. All three parameters influenced the ORFV recoveries siMembranesA Summary of Practical Considerations for the Application of the Steric Exclusion Chromatography for the Purification of the Orf Viral Vector.PMC9696199IGJ-ERDF-Program Hesse - React EU 20008790NAEU 2000879003EFKBW171Wolff MWLothert KPagallies FAmann ROrbay SEilts FfalseA Summary of Practical Considerations for the Application of the Steric Exclusion Chromatography for the Purification of the Orf Viral Vector.Steric exclusion chromatography (SXC) is a promising purification method for biological macromolecules such as the Orf virus (ORFV) vector. The method's principle is closely related to conventional polyethylene glycol (PEG) precipitation, repeatedly implementing membranes as porous chromatographic media. In the past decade, several purification tasks with SXC showed exceptionally high yields and a high impurity removal. However, the effect of varying process parameters, on the precipitation success and its limitations to SXC, is not yet well understood. For this reason, the precipitation behavior and SXC adaptation for ORFV were investigated for the PEG/ORFV contact time, the membranes pore size, and the type and concentration of ions. All three parameters influenced the ORFV recoveries si2022-01-01T00:00:00Z2022 Oct2025-04-19T14:43:36.045Z2025-04-19T14:43:36.045ZS-EPMC96961993636362510.3390/membranes12111070