biostudies-literatureZhao QModern Agro-industry Technology Research SystemShandong Modern Agricultural Technology & Industry SystemKey Research and Development Program of Shandong ProvinceAgricultural scientific and technological innovation project of Shandong Academy of Agricultural Sciences603https://www.ebi.ac.uk/biostudies/studies/S-EPMC9697364biostudies-literatureUnknown9(11)Rabbit astrovirus (RAstV) is a pathogen that causes diarrhea in rabbits, with high infection rate at various stages, which can often cause secondary or mixed infections with other pathogens, bringing great economic losses to the rabbit industry. In this study, 10 samples were collected from cases of rabbits with diarrhea on a rabbit meat farm in the Shandong area of China. The positive sample for astrovirus detected by RT-PCR was inoculated into an RK 13 cell line. A rabbit astrovirus strain named Z317 was successfully isolated, which produced an obvious cytopathic effect 48 h post-inoculation in the RK 13 cell line. The genome structure of this isolate was studied by high-throughput sequencing, showing that the Z317 strain had the highest similarity with the American strain TN/2208/2010, Veterinary sciencesThe Genomic and Genetic Evolution Analysis of Rabbit Astrovirus.PMC9697364CARS-43-G-72022CXGC010606SDAIT-21-06CXGC2022A25Jiang YLiu LZhao QTian YTan SSun HHuang BfalseThe Genomic and Genetic Evolution Analysis of Rabbit Astrovirus.Rabbit astrovirus (RAstV) is a pathogen that causes diarrhea in rabbits, with high infection rate at various stages, which can often cause secondary or mixed infections with other pathogens, bringing great economic losses to the rabbit industry. In this study, 10 samples were collected from cases of rabbits with diarrhea on a rabbit meat farm in the Shandong area of China. The positive sample for astrovirus detected by RT-PCR was inoculated into an RK 13 cell line. A rabbit astrovirus strain named Z317 was successfully isolated, which produced an obvious cytopathic effect 48 h post-inoculation in the RK 13 cell line. The genome structure of this isolate was studied by high-throughput sequencing, showing that the Z317 strain had the highest similarity with the American strain TN/2208/2010, 2022-01-01T00:00:00Z2022 Oct2026-04-08T11:06:39.959Z2024-10-15T07:23:17.542ZS-EPMC96973643635608010.3390/vetsci9110603