biostudies-literatureKim ALthe Ministry of SMEs and Startupsthe Rural Development Administration (RDA)the National Research Foundation (NRF)the Ministry of Trade, Industry and Energy3088https://www.ebi.ac.uk/biostudies/studies/S-EPMC9697443biostudies-literatureUnknown11(22)<i>Cannabis</i> (<i>Cannabis sativa</i> L.) is widely cultivated and studied for its psychoactive and medicinal properties. As the major cannabinoids are present in acidic forms in <i>Cannabis</i> plants, non-enzymatic processes, such as decarboxylation, are crucial for their conversion to neutral active cannabinoid forms. Herein, we detected the levels of cannabidivarin (CBDV), cannabidiol (CBD), cannabichromene (CBC), and Δ9-tetrahydrocannabinol (Δ9-THC) in the leaves and vegetative shoots of five commercial <i>Cannabis</i> cultivars using a combination of relatively simple extraction, decarboxylation, and high-performance liquid chromatography analyses. The CBDV, CBC, and Δ9-THC levels were 6.3-114.9, 34.4-187.2, and 57.6-407.4 μg/g, respectively, and the CBD levels were the highest, raPlants (Basel, Switzerland)Profiling Cannabinoid Contents and Expression Levels of Corresponding Biosynthetic Genes in Commercial <i>Cannabis</i> (<i>Cannabis sativa</i> L.) Cultivars.PMC96974432020R1I1A1A01072914PJ01652303200128921425151434Choi HWKim ALYun YJHong CHKim YCShim HJLee JHfalseProfiling Cannabinoid Contents and Expression Levels of Corresponding Biosynthetic Genes in Commercial <i>Cannabis</i> (<i>Cannabis sativa</i> L.) Cultivars.<i>Cannabis</i> (<i>Cannabis sativa</i> L.) is widely cultivated and studied for its psychoactive and medicinal properties. As the major cannabinoids are present in acidic forms in <i>Cannabis</i> plants, non-enzymatic processes, such as decarboxylation, are crucial for their conversion to neutral active cannabinoid forms. Herein, we detected the levels of cannabidivarin (CBDV), cannabidiol (CBD), cannabichromene (CBC), and Δ9-tetrahydrocannabinol (Δ9-THC) in the leaves and vegetative shoots of five commercial <i>Cannabis</i> cultivars using a combination of relatively simple extraction, decarboxylation, and high-performance liquid chromatography analyses. The CBDV, CBC, and Δ9-THC levels were 6.3-114.9, 34.4-187.2, and 57.6-407.4 μg/g, respectively, and the CBD levels were the highest, ra2022-01-01T00:00:00Z2022 Nov2026-06-21T03:11:27.281Z2025-04-06T23:22:31.476ZS-EPMC96974433643281710.3390/plants11223088