biostudies-literatureHazeena SHThe Ministry of Science and Technology funded this research, the Republic of ChinaMinistry of Science and Technology, the Republic of China7969https://www.ebi.ac.uk/biostudies/studies/S-EPMC9698347biostudies-literatureUnknown15(22)In fish processing, reducing the waste rate and increasing the economic value of products is an important issue for global environmental protection and resource sustainability. It has been discovered that cuttlefish bones can be an excellent resource for producing attractive amounts of chitin and chitosan. Therefore, this study optimized chitosan extraction conditions using response surface methodology (RSM) to establish application conditions suitable for industrial production and reducing environmental impact. In addition, Fourier-transform infrared spectroscopy (FTIR), ¹H NMR and scanning electron microscope (SEM) characteristics of extracted chitosan were evaluated. The optimum extraction conditions for chitosan from cuttlebone chitin were 12.5M NaOH, 6 h and 80 °C, and the Materials (Basel, Switzerland)Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone.PMC9698347111-2221-E-328-001-MY3Grant no. 111-2221-E-328 -001-MY3Grant no. 110-2622-E-992-012-111-2622-E-992-002-110-2622-E-992-012-Grant no. 111-2622-E-992-002-Lin TCLiu CSHsieh SLHou CYLi BHChang CIZeng JHShih MKHazeena SHfalseExtraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone.In fish processing, reducing the waste rate and increasing the economic value of products is an important issue for global environmental protection and resource sustainability. It has been discovered that cuttlefish bones can be an excellent resource for producing attractive amounts of chitin and chitosan. Therefore, this study optimized chitosan extraction conditions using response surface methodology (RSM) to establish application conditions suitable for industrial production and reducing environmental impact. In addition, Fourier-transform infrared spectroscopy (FTIR), ¹H NMR and scanning electron microscope (SEM) characteristics of extracted chitosan were evaluated. The optimum extraction conditions for chitosan from cuttlebone chitin were 12.5M NaOH, 6 h and 80 °C, and the 2022-01-01T00:00:00Z2022 Nov2026-04-08T05:43:48.689Z2024-11-20T23:33:47.19ZS-EPMC96983473643145610.3390/ma15227969