biostudies-literatureUnknown23(22)Yang HBasical Science Research Fund in Sichuan Key Laboratory of Ecological Restoration and Conservation for Forest and WetlandKey Research and Development Project of Sichuan ProvinceForest Ecosystem Improvement in the Upper Reaches of Yangtze River Basin ProgramNational Undergraduate Training Program for Innovation and EntrepreneurshipNatural Science Foundation of Sichuan ProvincePhoebe hui is an extremely valuable tree that is the main source of the fragrant golden-thread nanmu wood. Although the fragrance of wood has been investigated in several trees, the potential substances and gene regulation mechanisms that are involved in fragrance formation are poorly understood. Here, three radial tissues, sapwood (SW), heartwood (HW), and the transition zone (TZ) in between them, were compared via integrative physiological, volatile-metabolomic, and transcriptomic analyses to identify the key metabolites and regulatory mechanisms involved in fragrance formation. During heartwood formation, gradual starch grain loss was accompanied by the deposition of lipids and extractives in the cell lumen. Extracts of terpenoids were synthesized and accumulated in the heartwood, incluInternational journal of molecular scienceshttps://www.ebi.ac.uk/biostudies/studies/S-EPMC9698381biostudies-literatureIntegrated Transcriptomic, Metabolomic, and Physiological Analyses Reveal New Insights into Fragrance Formation in the Heartwood of Phoebe huiPMC96983815102012020384672021YFYZ00322022SKT4-022022NSFSC1062202210626011Yang HZhang FGu YLiu MChen LWang FWan XJiang YZhu PGuo HHuang XAn WPeng JfalseIntegrated Transcriptomic, Metabolomic, and Physiological Analyses Reveal New Insights into Fragrance Formation in the Heartwood of Phoebe huiPhoebe hui is an extremely valuable tree that is the main source of the fragrant golden-thread nanmu wood. Although the fragrance of wood has been investigated in several trees, the potential substances and gene regulation mechanisms that are involved in fragrance formation are poorly understood. Here, three radial tissues, sapwood (SW), heartwood (HW), and the transition zone (TZ) in between them, were compared via integrative physiological, volatile-metabolomic, and transcriptomic analyses to identify the key metabolites and regulatory mechanisms involved in fragrance formation. During heartwood formation, gradual starch grain loss was accompanied by the deposition of lipids and extractives in the cell lumen. Extracts of terpenoids were synthesized and accumulated in the heartwood, inclu2022-01-01T00:00:00Z2022 Nov2025-04-18T13:19:50.12Z2025-04-06T22:56:40.474ZS-EPMC9698381