biostudies-literatureUnknown43(6)Yang XCAs a transcription factor of the Pit-Oct-Unc (POU) domain family, octamer-binding transcription factor 6 ( <i>OCT6</i>) participates in various aspects of stem cell development and differentiation. At present, however, its role in porcine-induced pluripotent stem cells (piPSCs) remains unclear. Here, we explored the function of <i>OCT6</i> in piPSCs. We found that piPSCs overexpressing <i>OCT6</i> maintained colony morphology and pluripotency under differentiation conditions, with a similar gene expression pattern to that of non-differentiated piPSCs. Functional analysis revealed that <i>OCT6</i> attenuated the adverse effects of extracellular signal-regulated kinase (ERK) signaling pathway inhibition on piPSC pluripotency by activating phosphatidylinositol 3-kinase-protein kinase B (PI3K-AKT) signaling activity. Our research sheds new light on the mechanism by which <i>OCT6</i> promotes PSC maintenance.Zoological research911-922https://www.ebi.ac.uk/biostudies/studies/S-EPMC9700490biostudies-literature<i>OCT6</i> inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation.PMC9700490Yang XCShen QYHua JLPeng SWu XLLi WHWu XJLi YXfalse<i>OCT6</i> inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation.As a transcription factor of the Pit-Oct-Unc (POU) domain family, octamer-binding transcription factor 6 ( <i>OCT6</i>) participates in various aspects of stem cell development and differentiation. At present, however, its role in porcine-induced pluripotent stem cells (piPSCs) remains unclear. Here, we explored the function of <i>OCT6</i> in piPSCs. We found that piPSCs overexpressing <i>OCT6</i> maintained colony morphology and pluripotency under differentiation conditions, with a similar gene expression pattern to that of non-differentiated piPSCs. Functional analysis revealed that <i>OCT6</i> attenuated the adverse effects of extracellular signal-regulated kinase (ERK) signaling pathway inhibition on piPSC pluripotency by activating phosphatidylinositol 3-kinase-protein kinase B (PI3K-AKT) signaling activity. Our research sheds new light on the mechanism by which <i>OCT6</i> promotes PSC maintenance.2022-01-01T00:00:00Z2022 Nov2025-04-26T09:44:05.161Z2025-04-06T13:06:58.862ZS-EPMC97004903605256110.24272/j.issn.2095-8137.2022.220