biostudies-literatureCorrales EDeutsches Krebsforschungszentrum (DKFZ)Bundesministerium für Bildung und ForschungDeutsche ForschungsgemeinschaftDeutschen Konsortium für Translationale Krebsforschung187https://www.ebi.ac.uk/biostudies/studies/S-EPMC9700886biostudies-literatureUnknown20(1)<h4>Background</h4>Current therapeutic management of advanced melanoma patients largely depends on their BRAF mutation status. However, the vast heterogeneity of the tumors hampers the success of therapies targeting the MAPK/ERK pathway alone. Dissecting this heterogeneity will contribute to identifying key players in the oncogenic progression to tailor more effective therapies.<h4>Methods</h4>We performed a comprehensive molecular and phenotypic characterization of a panel of patient-derived BRAF^V600E-positive melanoma cell lines. Transcriptional profiling was used to identify groups of coregulated genes whose expression relates to an increased migratory potential and a higher resistance.<h4>Results</h4>A decrease in sensitivity to MAPK/ERK pathway inhibition with vemurafenib oCell communication and signaling : CCSPI3K/AKT signaling allows for MAPK/ERK pathway independency mediating dedifferentiation-driven treatment resistance in melanoma.PMC9700886GRK2344FKZ 01ZZ1801BMertes RKowar SCorrales ELemke SBoerries MMunch JLevit-Zerdoun ELehmann AMetzger PfalsePI3K/AKT signaling allows for MAPK/ERK pathway independency mediating dedifferentiation-driven treatment resistance in melanoma.<h4>Background</h4>Current therapeutic management of advanced melanoma patients largely depends on their BRAF mutation status. However, the vast heterogeneity of the tumors hampers the success of therapies targeting the MAPK/ERK pathway alone. Dissecting this heterogeneity will contribute to identifying key players in the oncogenic progression to tailor more effective therapies.<h4>Methods</h4>We performed a comprehensive molecular and phenotypic characterization of a panel of patient-derived BRAF^V600E-positive melanoma cell lines. Transcriptional profiling was used to identify groups of coregulated genes whose expression relates to an increased migratory potential and a higher resistance.<h4>Results</h4>A decrease in sensitivity to MAPK/ERK pathway inhibition with vemurafenib o2022-01-01T00:00:00Z2022 Nov2025-04-19T20:36:30.31Z2025-02-19T04:36:57.966ZS-EPMC97008863643461610.1186/s12964-022-00989-y