{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shetty RM"],"funding":["Division of Computing and Communication Foundations","Arizona Biomedical Research Commission","NIH Office of the Director","NIAID NIH HHS","Office of Naval Research Global","Division of Civil, Mechanical and Manufacturing Innovation"],"pagination":["11441-11450"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9701110"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(7)"],"pubmed_abstract":["Large-scale nanoarrays of single biomolecules enable high-throughput assays while unmasking the underlying heterogeneity within ensemble populations. Until recently, creating such grids which combine the advantages of microarrays and single-molecule experiments (SMEs) has been particularly challenging due to the mismatch between the size of these molecules and the resolution of top-down fabrication techniques. DNA origami placement (DOP) combines two powerful techniques to address this issue: (i) DNA origami, which provides a ∼100 nm self-assembled template for single-molecule organization with 5 nm resolution and (ii) top-down lithography, which patterns these DNA nanostructures, transforming them into functional nanodevices via large-scale integration with arbitrary substrates. Presently, this technique relies on state-of-the-art infrastructure and highly trained personnel, making it prohibitively expensive for researchers. Here, we introduce a cleanroom-free, $1 benchtop technique to create meso-to-macro-scale DNA origami nanoarrays using self-assembled colloidal nanoparticles, thereby circumventing the need for top-down fabrication. We report a maximum yield of 74%, 2-fold higher than the statistical limit of 37% imposed on non-specific molecular loading alternatives. Furthermore, we provide a proof-of-principle for the ability of this nanoarray platform to transform traditionally low-throughput, stochastic, single-molecule assays into high-throughput, deterministic ones, without compromising data quality. Our approach has the potential to democratize single-molecule nanoarrays and demonstrates their utility as a tool for biophysical assays and diagnostics."],"journal":["ACS nano"],"pubmed_title":["Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement."],"pmcid":["PMC9701110"],"funding_grant_id":["CCF-1317694","CMMI-1636364","N00014-17-1-2610","DP2 AI144247","1DP2AI144247","ADHS17-00007401","N00014-18-1-2649"],"pubmed_authors":["Rothemund PWK","Gopinath A","Shetty RM","Brady SR","Hariadi RF"],"additional_accession":[]},"is_claimable":false,"name":"Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement.","description":"Large-scale nanoarrays of single biomolecules enable high-throughput assays while unmasking the underlying heterogeneity within ensemble populations. Until recently, creating such grids which combine the advantages of microarrays and single-molecule experiments (SMEs) has been particularly challenging due to the mismatch between the size of these molecules and the resolution of top-down fabrication techniques. DNA origami placement (DOP) combines two powerful techniques to address this issue: (i) DNA origami, which provides a ∼100 nm self-assembled template for single-molecule organization with 5 nm resolution and (ii) top-down lithography, which patterns these DNA nanostructures, transforming them into functional nanodevices via large-scale integration with arbitrary substrates. Presently, this technique relies on state-of-the-art infrastructure and highly trained personnel, making it prohibitively expensive for researchers. Here, we introduce a cleanroom-free, $1 benchtop technique to create meso-to-macro-scale DNA origami nanoarrays using self-assembled colloidal nanoparticles, thereby circumventing the need for top-down fabrication. We report a maximum yield of 74%, 2-fold higher than the statistical limit of 37% imposed on non-specific molecular loading alternatives. Furthermore, we provide a proof-of-principle for the ability of this nanoarray platform to transform traditionally low-throughput, stochastic, single-molecule assays into high-throughput, deterministic ones, without compromising data quality. Our approach has the potential to democratize single-molecule nanoarrays and demonstrates their utility as a tool for biophysical assays and diagnostics.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-04-25T20:57:04.914Z","creation":"2025-04-06T08:33:14.086Z"},"accession":"S-EPMC9701110","cross_references":{"pubmed":["34228915"],"doi":["10.1021/acsnano.1c01150"]}}