{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(11)"],"submitter":["Li Y"],"pubmed_abstract":["The gut microbiome is essential for human health. Mouse microbiota models, including gnotobiotic mice, are the most prominent tools to elucidate the functions of gut bacteria. Here, we propose a targeted-bacterium-depleted (TBD) model using lytic bacteriophage to selectively deplete gut bacterium of healthy or otherwise defined mice. These phage-treated animals should have a near-complete spectrum of gut bacteria except for the depleted bacterium. To prove the concept, we employed <i>Escherichia coli</i>-specific phage T7 to repress <i>E. coli</i> in the healthy mice. Our results showed that the <i>E. coli-</i>depleted mice exhibited bravery-like behaviors, correlated to the presence of <i>E. coli</i> rather than the equilibrium among gut bacteria. Thus, we demonstrate that the TBD model is a powerful tool to elucidate the function of a specific bacterial species within a near-intact gut microbiota environment and complements gnotobiotic mice models."],"journal":["Cell reports methods"],"pagination":["100324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9701607"],"repository":["biostudies-literature"],"pubmed_title":["Bacteriophages allow selective depletion of gut bacteria to produce a targeted-bacterium-depleted mouse model."],"pmcid":["PMC9701607"],"pubmed_authors":["Pan S","Hu Z","Zhu F","Ma X","Wang H","Yu J","Liu X","Li Y","Yang Z","Li T","Liu B","Wang Y","Wang Z","Barritt JD"],"additional_accession":[]},"is_claimable":false,"name":"Bacteriophages allow selective depletion of gut bacteria to produce a targeted-bacterium-depleted mouse model.","description":"The gut microbiome is essential for human health. Mouse microbiota models, including gnotobiotic mice, are the most prominent tools to elucidate the functions of gut bacteria. Here, we propose a targeted-bacterium-depleted (TBD) model using lytic bacteriophage to selectively deplete gut bacterium of healthy or otherwise defined mice. These phage-treated animals should have a near-complete spectrum of gut bacteria except for the depleted bacterium. To prove the concept, we employed <i>Escherichia coli</i>-specific phage T7 to repress <i>E. coli</i> in the healthy mice. Our results showed that the <i>E. coli-</i>depleted mice exhibited bravery-like behaviors, correlated to the presence of <i>E. coli</i> rather than the equilibrium among gut bacteria. Thus, we demonstrate that the TBD model is a powerful tool to elucidate the function of a specific bacterial species within a near-intact gut microbiota environment and complements gnotobiotic mice models.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-21T14:28:18.951Z","creation":"2025-04-21T14:28:18.951Z"},"accession":"S-EPMC9701607","cross_references":{"pubmed":["36452872"],"doi":["10.1016/j.crmeth.2022.100324"]}}