biostudies-literatureUnknown12Xue MCentral Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery SciencesNational Key Research and Development Program of ChinaThe influence of dietary probiotic <i>Bacillus amyloliquefaciens</i> on the growth performance, digestive enzyme activity, immune parameters and disease resistance of yellow catfish (<i>Pelteobagrus fulvidraco</i>) was evaluated. Commercial diet (C) or diet containing 10⁶ cfu/g <i>B. amyloliquefaciens</i> (T) was fed for 4 weeks, and final weight (FW), specific growth rate (SGR) and feed conversion ratio (FCR) were improved (<i>p<</i>0.05) in the T group. Dietary <i>B. amyloliquefaciens</i> increased protease and amylase activities in the digestive tract after 2 and 4 weeks, respectively. Respiratory burst (RB), plasma lysozyme (LZM) activity, total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity were also elevated (<i>p<</i>0.05). Immune-related genes signal transducer and activator of transcription 1 (STATA-1), immunoglobulin M (IgM) and C-type lectin (CTL) were upregulated (<i>p<</i>0.05), but interleukin-1 β (IL-1β) was not (<i>p ></i>0.05). Intestinal microbiota analysis showed that the community structure was significantly different between the two groups; the relative abundance of <i>Cetobacterium</i> was increased but <i>Plesiomonas</i> was decreased in T. Moreover, challenge tests showed that the resistance of fish fed <i>B. amyloliquefaciens</i> against <i>Aeromonas veronii</i> and <i>Edwardsiella ictaluri</i> was significantly enhanced (<i>p<</i>0.05). In conclusion, dietary supplementation of <i>B. amyloliquefaciens</i> can effectively improve the growth performance, digestive enzyme activity, immune responses, intestinal microbiota composition and disease resistance of yellow catfish.Frontiers in cellular and infection microbiology1047351https://www.ebi.ac.uk/biostudies/studies/S-EPMC9702064biostudies-literatureEffects of dietary <i>Bacillus amyloliquefaciens</i> on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow catfish, <i>Pelteobagrus fulvidraco</i>.PMC9702064Li YFan YHong YXu CXue MZhou YMeng YJiang NWu YLiu WfalseEffects of dietary <i>Bacillus amyloliquefaciens</i> on the growth, immune responses, intestinal microbiota composition and disease resistance of yellow catfish, <i>Pelteobagrus fulvidraco</i>.The influence of dietary probiotic <i>Bacillus amyloliquefaciens</i> on the growth performance, digestive enzyme activity, immune parameters and disease resistance of yellow catfish (<i>Pelteobagrus fulvidraco</i>) was evaluated. Commercial diet (C) or diet containing 10⁶ cfu/g <i>B. amyloliquefaciens</i> (T) was fed for 4 weeks, and final weight (FW), specific growth rate (SGR) and feed conversion ratio (FCR) were improved (<i>p<</i>0.05) in the T group. Dietary <i>B. amyloliquefaciens</i> increased protease and amylase activities in the digestive tract after 2 and 4 weeks, respectively. Respiratory burst (RB), plasma lysozyme (LZM) activity, total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity were also elevated (<i>p<</i>0.05). Immune-related genes signal transducer and activator of transcription 1 (STATA-1), immunoglobulin M (IgM) and C-type lectin (CTL) were upregulated (<i>p<</i>0.05), but interleukin-1 β (IL-1β) was not (<i>p ></i>0.05). Intestinal microbiota analysis showed that the community structure was significantly different between the two groups; the relative abundance of <i>Cetobacterium</i> was increased but <i>Plesiomonas</i> was decreased in T. Moreover, challenge tests showed that the resistance of fish fed <i>B. amyloliquefaciens</i> against <i>Aeromonas veronii</i> and <i>Edwardsiella ictaluri</i> was significantly enhanced (<i>p<</i>0.05). In conclusion, dietary supplementation of <i>B. amyloliquefaciens</i> can effectively improve the growth performance, digestive enzyme activity, immune responses, intestinal microbiota composition and disease resistance of yellow catfish.2022-01-01T00:00:00Z20222025-04-19T22:49:33.351Z2025-04-19T22:49:33.351ZS-EPMC97020643645230010.3389/fcimb.2022.1047351