biostudies-literatureUnknown13Shi HThree new antibacterial spirooxindole alkaloids, spirobrefeldins A-C (1-3), together with four known analogs, spirotryprostatin M (4), spirotryprostatin G (5), 12β-hydroxyverruculogen TR-2 (6), and 12α-hydroxyverruculogen TR-2 (7), were isolated from terrestrial fungus <i>Penicillium brefeldianum.</i> All the new compounds were elucidated extensively by the interpretation of their NMR (1D and 2D) spectra and high-resolution mass data, and their absolute configurations were determined by computational chemistry and CD spectra. The absolute configurations of spiro carbon C-2 in spirotryprostatin G (5) and spirotryprostatin C in literature were reported as <i>S</i>, which were revised to <i>R</i> based on experimental and calculated CD spectra. All the compounds were evaluated for their antimFrontiers in microbiology1046099https://www.ebi.ac.uk/biostudies/studies/S-EPMC9702524biostudies-literatureAntibacterial spirooxindole alkaloids from <i>Penicillium brefeldianum</i> inhibit dimorphism of pathogenic smut fungi.PMC9702524Jiang JHe FJie LZhang HJiang HSu ZLiu DShi HfalseAntibacterial spirooxindole alkaloids from <i>Penicillium brefeldianum</i> inhibit dimorphism of pathogenic smut fungi.Three new antibacterial spirooxindole alkaloids, spirobrefeldins A-C (1-3), together with four known analogs, spirotryprostatin M (4), spirotryprostatin G (5), 12β-hydroxyverruculogen TR-2 (6), and 12α-hydroxyverruculogen TR-2 (7), were isolated from terrestrial fungus <i>Penicillium brefeldianum.</i> All the new compounds were elucidated extensively by the interpretation of their NMR (1D and 2D) spectra and high-resolution mass data, and their absolute configurations were determined by computational chemistry and CD spectra. The absolute configurations of spiro carbon C-2 in spirotryprostatin G (5) and spirotryprostatin C in literature were reported as <i>S</i>, which were revised to <i>R</i> based on experimental and calculated CD spectra. All the compounds were evaluated for their antim2022-01-01T00:00:00Z20222025-04-04T12:02:09.299Z2025-02-19T03:00:06.176ZS-EPMC97025243645292210.3389/fmicb.2022.1046099