{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(52)"],"submitter":["Wang H"],"pubmed_abstract":["In this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH). The probe MITO-PQDNs could react rapidly (20 min) with GSH in PBS buffer and exhibited a strong fluorescence signal (586 nm) as well as a significant Stokes shift (200 nm). Moreover, MITO-PQDNs could quantitatively detect GSH with high sensitivity (LOD = 253 nM). Meanwhile, MITO-PQDNs possessed favorable biocompatibility and could detect both endogenous and exogenous GSH in MCF-7 cells. Above all, MITO-PQDNs enabled the detection of fluctuations in mitochondrial GSH concentrations during oxidative stress. In this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH)."],"journal":["RSC advances"],"pagination":["33922-33927"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9703030"],"repository":["biostudies-literature"],"pubmed_title":["A water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2ra04732b"],"pmcid":["PMC9703030"],"pubmed_authors":["Ding X","Jin X","Wang H","Zhang L","Chang J","Tian P"],"additional_accession":[]},"is_claimable":false,"name":"A water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2ra04732b","description":"In this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH). The probe MITO-PQDNs could react rapidly (20 min) with GSH in PBS buffer and exhibited a strong fluorescence signal (586 nm) as well as a significant Stokes shift (200 nm). Moreover, MITO-PQDNs could quantitatively detect GSH with high sensitivity (LOD = 253 nM). Meanwhile, MITO-PQDNs possessed favorable biocompatibility and could detect both endogenous and exogenous GSH in MCF-7 cells. Above all, MITO-PQDNs enabled the detection of fluctuations in mitochondrial GSH concentrations during oxidative stress. In this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH).","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-29T11:06:43.336Z","creation":"2025-02-18T23:49:01.044Z"},"accession":"S-EPMC9703030","cross_references":{}}