biostudies-literatureUnknown12(52)Wang HIn this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH). The probe MITO-PQDNs could react rapidly (20 min) with GSH in PBS buffer and exhibited a strong fluorescence signal (586 nm) as well as a significant Stokes shift (200 nm). Moreover, MITO-PQDNs could quantitatively detect GSH with high sensitivity (LOD = 253 nM). Meanwhile, MITO-PQDNs possessed favorable biocompatibility and could detect both endogenous and exogenous GSH in MCF-7 cells. Above all, MITO-PQDNs enabled the detection of fluctuations in mitochondrial GSH concentrations during oxidative stress. In this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH).RSC advances33922-33927https://www.ebi.ac.uk/biostudies/studies/S-EPMC9703030biostudies-literatureA water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2ra04732bPMC9703030Ding XJin XWang HZhang LChang JTian PfalseA water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2ra04732bIn this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH). The probe MITO-PQDNs could react rapidly (20 min) with GSH in PBS buffer and exhibited a strong fluorescence signal (586 nm) as well as a significant Stokes shift (200 nm). Moreover, MITO-PQDNs could quantitatively detect GSH with high sensitivity (LOD = 253 nM). Meanwhile, MITO-PQDNs possessed favorable biocompatibility and could detect both endogenous and exogenous GSH in MCF-7 cells. Above all, MITO-PQDNs enabled the detection of fluctuations in mitochondrial GSH concentrations during oxidative stress. In this research, we constructed a styrylpyridine derivative-based fluorescent probe MITO-PQDNs to monitor mitochondrial glutathione (GSH).2022-01-01T00:00:00Z2022 Nov2025-04-29T11:06:43.336Z2025-02-18T23:49:01.044ZS-EPMC9703030