biostudies-literatureUnknown13Xia X<h4>Objective</h4>Diarrhea-predominant irritable bowel syndrome (IBS-D) is a recurrent and common disease featuring dysbiotic intestinal microbiota, with limited treatments. Si-Jun-Zi Decoction (SJZD), a classic Chinese prescription, has been extensively used for IBS-D. This work aimed to explore the <i>ex vivo</i> interactions of SJZD and IBS-D's intestinal microbiota.<h4>Methods</h4>Five samples of intestinal microbiota collected from IBS-D volunteers and five age-matched healthy controls were recruited from the Affiliated Hospital, Chengdu University of Traditional Chinese Medicine (TCM). A representative mixture of intestinal microbiota was composed of an equal proportion of these fecal samples. To simulate the clinical interaction, this microbiota was cocultivated with SJZD at clinicaFrontiers in endocrinology1053103https://www.ebi.ac.uk/biostudies/studies/S-EPMC9704418biostudies-literatureCocultivation of Chinese prescription and intestine microbiota: SJZD alleviated the major symptoms of IBS-D subjects by tuning neurotransmitter metabolism.PMC9704418Xia XDing WChen QXie YDing DWang XWang YWang ZXu YfalseCocultivation of Chinese prescription and intestine microbiota: SJZD alleviated the major symptoms of IBS-D subjects by tuning neurotransmitter metabolism.<h4>Objective</h4>Diarrhea-predominant irritable bowel syndrome (IBS-D) is a recurrent and common disease featuring dysbiotic intestinal microbiota, with limited treatments. Si-Jun-Zi Decoction (SJZD), a classic Chinese prescription, has been extensively used for IBS-D. This work aimed to explore the <i>ex vivo</i> interactions of SJZD and IBS-D's intestinal microbiota.<h4>Methods</h4>Five samples of intestinal microbiota collected from IBS-D volunteers and five age-matched healthy controls were recruited from the Affiliated Hospital, Chengdu University of Traditional Chinese Medicine (TCM). A representative mixture of intestinal microbiota was composed of an equal proportion of these fecal samples. To simulate the clinical interaction, this microbiota was cocultivated with SJZD at clinica2022-01-01T00:00:00Z20222026-05-29T18:57:23.155Z2024-11-21T01:22:36.986ZS-EPMC97044183645232710.3389/fendo.2022.1053103