{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stanciu SG"],"funding":["EC | Horizon 2020 Framework Programme (H2020)","Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (UEFISCDI)","EC | Horizon 2020 Framework Programme","Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii"],"pagination":["e2214662119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9704717"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(47)"],"pubmed_abstract":["Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Super-resolution re-scan second harmonic generation microscopy."],"pmcid":["PMC9704717"],"funding_grant_id":["PN-III-P2-2.1-PED-2019-1666","RO-NO-2019-0601","777222 (1052)"],"pubmed_authors":["Costache M","Stenmark HA","Tranca DE","Cherian A","Hristu R","Eftimie L","Stanciu GA","Dumitru A","Tark-Dame M","Manders H","Stanciu SG","Manders EMM"],"additional_accession":[]},"is_claimable":false,"name":"Super-resolution re-scan second harmonic generation microscopy.","description":"Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-21T21:25:08.266Z","creation":"2025-04-05T18:20:39.023Z"},"accession":"S-EPMC9704717","cross_references":{"pubmed":["36375085"],"doi":["10.1073/pnas.2214662119"]}}