biostudies-literatureStanciu SGEC | Horizon 2020 Framework Programme (H2020)Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (UEFISCDI)EC | Horizon 2020 Framework ProgrammeUnitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovariie2214662119https://www.ebi.ac.uk/biostudies/studies/S-EPMC9704717biostudies-literatureUnknown119(47)Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.Proceedings of the National Academy of Sciences of the United States of AmericaSuper-resolution re-scan second harmonic generation microscopy.PMC9704717PN-III-P2-2.1-PED-2019-1666RO-NO-2019-0601777222 (1052)Costache MStenmark HATranca DECherian AHristu REftimie LStanciu GADumitru ATark-Dame MManders HStanciu SGManders EMMfalseSuper-resolution re-scan second harmonic generation microscopy.Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.2022-01-01T00:00:00Z2022 Nov2025-04-21T21:25:08.266Z2025-04-05T18:20:39.023ZS-EPMC97047173637508510.1073/pnas.2214662119