{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Reay MK"],"funding":["Natural Environment Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["369-389"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9705487"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["480(1-2)"],"pubmed_abstract":["<h4>Purpose</h4>Nitrogen (N) transfer from white clover (<i>Trifolium repens</i> cv.) to ryegrass (<i>Lolium perenne</i> cv.) has the potential to meet ryegrass N requirements. This study aimed to quantify N transfer in a mixed pasture and investigate the influence of the microbial community and land management on N transfer.<h4>Methods</h4>Split root <sup>15</sup>N-labelling of clover quantified N transfer to ryegrass via exudation, microbial assimilation, decomposition, defoliation and soil biota. Incorporation into the microbial protein pool was determined using compound-specific <sup>15</sup>N-stable isotope probing approaches.<h4>Results</h4>N transfer to ryegrass and soil microbial protein in the model system was relatively small, with one-third arising from root exudation. N transfer to ryegrass increased with no microbial competition but soil microbes also increased N transfer via shoot decomposition. Addition of mycorrhizal fungi did not alter N transfer, due to the source-sink nature of this pathway, whilst weevil grazing on roots decreased microbial N transfer. N transfer was bidirectional, and comparable on a short-term scale.<h4>Conclusions</h4>N transfer was low in a model young pasture established from soil from a permanent grassland with long-term N fertilisation. Root exudation and decomposition were major N transfer pathways. N transfer was influenced by soil biota (weevils, mycorrhizae) and land management (e.g. grazing). Previous land management and the role of the microbial community in N transfer must be considered when determining the potential for N transfer to ryegrass.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s11104-022-05585-0."],"journal":["Plant and soil"],"pubmed_title":["Mechanisms of nitrogen transfer in a model clover-ryegrass pasture: a <sup>15</sup>N-tracer approach."],"pmcid":["PMC9705487"],"funding_grant_id":["SWBio DTP PhD scholarship","BBS/E/C/000J0100","NE/S011587/1"],"pubmed_authors":["Kuhl A","Reay MK","Cardenas LM","Murray PJ","Evershed RP","Dungait JAJ","Pears KA","Bull ID"],"additional_accession":[]},"is_claimable":false,"name":"Mechanisms of nitrogen transfer in a model clover-ryegrass pasture: a <sup>15</sup>N-tracer approach.","description":"<h4>Purpose</h4>Nitrogen (N) transfer from white clover (<i>Trifolium repens</i> cv.) to ryegrass (<i>Lolium perenne</i> cv.) has the potential to meet ryegrass N requirements. This study aimed to quantify N transfer in a mixed pasture and investigate the influence of the microbial community and land management on N transfer.<h4>Methods</h4>Split root <sup>15</sup>N-labelling of clover quantified N transfer to ryegrass via exudation, microbial assimilation, decomposition, defoliation and soil biota. Incorporation into the microbial protein pool was determined using compound-specific <sup>15</sup>N-stable isotope probing approaches.<h4>Results</h4>N transfer to ryegrass and soil microbial protein in the model system was relatively small, with one-third arising from root exudation. N transfer to ryegrass increased with no microbial competition but soil microbes also increased N transfer via shoot decomposition. Addition of mycorrhizal fungi did not alter N transfer, due to the source-sink nature of this pathway, whilst weevil grazing on roots decreased microbial N transfer. N transfer was bidirectional, and comparable on a short-term scale.<h4>Conclusions</h4>N transfer was low in a model young pasture established from soil from a permanent grassland with long-term N fertilisation. Root exudation and decomposition were major N transfer pathways. N transfer was influenced by soil biota (weevils, mycorrhizae) and land management (e.g. grazing). Previous land management and the role of the microbial community in N transfer must be considered when determining the potential for N transfer to ryegrass.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s11104-022-05585-0.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2024-11-10T02:41:52.367Z","creation":"2024-11-10T02:41:52.367Z"},"accession":"S-EPMC9705487","cross_references":{"pubmed":["36466744"],"doi":["10.1007/s11104-022-05585-0"]}}