biostudies-literatureUnknown62(4)Zhang JIn <i>Bacillus</i>, the spore formation process is associated with the synthesis and release of secondary metabolites. A large number of studies have been conducted to systematically elucidate the pathways and mechanisms of spore formation. However, there are no studies have explored the relationship between secondary metabolites and spores. In this study, we investigated the relationship between its secondary metabolite bacillomycin D (BD) and spores using the simpler dipicolonic acid fluorimetry assay for spore counting in <i>Bacillus amyloliquefaciens</i> fmbJ. Our results showed that BD could promote the spore formation of <i>B. amyloliquefaciens</i> fmbJ and had a synergistic effect with certain concentrations of Mn²⁺. When 15.6 mg/L of BD and 1 mM of Mn²⁺ were aIndian journal of microbiology531-539https://www.ebi.ac.uk/biostudies/studies/S-EPMC9705635biostudies-literaturePromoted Spore Formation of <i>Bacillus amyloliquefaciens</i> fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn².PMC9705635Li XZhang JLu YLuo XPang XSun JfalsePromoted Spore Formation of <i>Bacillus amyloliquefaciens</i> fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn².In <i>Bacillus</i>, the spore formation process is associated with the synthesis and release of secondary metabolites. A large number of studies have been conducted to systematically elucidate the pathways and mechanisms of spore formation. However, there are no studies have explored the relationship between secondary metabolites and spores. In this study, we investigated the relationship between its secondary metabolite bacillomycin D (BD) and spores using the simpler dipicolonic acid fluorimetry assay for spore counting in <i>Bacillus amyloliquefaciens</i> fmbJ. Our results showed that BD could promote the spore formation of <i>B. amyloliquefaciens</i> fmbJ and had a synergistic effect with certain concentrations of Mn²⁺. When 15.6 mg/L of BD and 1 mM of Mn²⁺ were a2022-01-01T00:00:00Z2022 Dec2025-04-22T13:18:16.047Z2025-04-06T00:33:17.2ZS-EPMC97056353645822310.1007/s12088-022-01026-9