{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["84(11)"],"submitter":["Okamoto M"],"pubmed_abstract":["American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness."],"journal":["The Journal of veterinary medical science"],"pagination":["1453-1456"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9705809"],"repository":["biostudies-literature"],"pubmed_title":["Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays."],"pmcid":["PMC9705809"],"pubmed_authors":["Takamatsu D","Okamoto M","Sugimoto I","Furuya H"],"additional_accession":[]},"is_claimable":false,"name":"Detection of macrolide resistance genes, ermC and ermB, in Japanese honey using real-time PCR assays.","description":"American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-28T01:11:16.068Z","creation":"2025-04-19T22:50:03.197Z"},"accession":"S-EPMC9705809","cross_references":{"pubmed":["36123020"],"doi":["10.1292/jvms.22-0307"]}}