biostudies-literatureUnknown2022Koontanatechanon A<h4>Background and aims</h4>Hyperlipidemia is a risk factor for cardiovascular diseases. This study is aimed at investigating the effects of consuming omega-3-rich pork lard on the serum lipid profile and gut microbiome of the mice model.<h4>Methods and results</h4>We divided 23 C57BL/6NJ males (16-week-old) into 3 groups, and each group received either a control diet, a high-fat diet of coconut oil (coconut oil), or a high-fat diet of omega-3-rich pork lard (omega lard) for 28 days. Thereafter, fasting serum lipids and fecal microbiomes were analyzed. The serum cholesterol, triglyceride, and LDL levels of the omega lard-treated group were significantly reduced compared to the coconut oil-treated group (<i>P</i> < 0.05). However, the microbiome analysis revealed a significant increase in the abundance of <i>Lachnospiraceae</i> in the omega lard-treated group compared to the coconut oil-treated group (<i>P</i> < 0.05). Furthermore, Spearman's correlation analysis revealed that the increased serum lipid content was positively correlated with the abundance of <i>Bacteroidaceae</i> (<i>P</i> < 0.05) and negatively correlated with the abundance of <i>Lachnospiraceae</i> (<i>P</i> < 0.05).<h4>Conclusions</h4>These findings suggested that omega-3-rich pork lard altered the serum lipid profile and gut microbiome in the mice model. <i>Practical Application</i>. The excellent protection offered by omega-3-rich pork lard against hyperlipidemia indicated that pork lard could be used as alternative cooking oil for health-conscious individuals. It could also be introduced as a functional ingredient for patients with hyperlipidemia.International journal of food science9269968https://www.ebi.ac.uk/biostudies/studies/S-EPMC9708346biostudies-literatureEffects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice.PMC9708346Jariyahatthakij PLeksrisompong PKhorporn TPrasopdee SWongphatcharachai MSriyakul KKeattisin BRoytrakul SParnsen WNonthabenjawan NPawa KKSrichana PThitapakorn VKoontanatechanon AfalseEffects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice.<h4>Background and aims</h4>Hyperlipidemia is a risk factor for cardiovascular diseases. This study is aimed at investigating the effects of consuming omega-3-rich pork lard on the serum lipid profile and gut microbiome of the mice model.<h4>Methods and results</h4>We divided 23 C57BL/6NJ males (16-week-old) into 3 groups, and each group received either a control diet, a high-fat diet of coconut oil (coconut oil), or a high-fat diet of omega-3-rich pork lard (omega lard) for 28 days. Thereafter, fasting serum lipids and fecal microbiomes were analyzed. The serum cholesterol, triglyceride, and LDL levels of the omega lard-treated group were significantly reduced compared to the coconut oil-treated group (<i>P</i> < 0.05). However, the microbiome analysis revealed a significant increase in the abundance of <i>Lachnospiraceae</i> in the omega lard-treated group compared to the coconut oil-treated group (<i>P</i> < 0.05). Furthermore, Spearman's correlation analysis revealed that the increased serum lipid content was positively correlated with the abundance of <i>Bacteroidaceae</i> (<i>P</i> < 0.05) and negatively correlated with the abundance of <i>Lachnospiraceae</i> (<i>P</i> < 0.05).<h4>Conclusions</h4>These findings suggested that omega-3-rich pork lard altered the serum lipid profile and gut microbiome in the mice model. <i>Practical Application</i>. The excellent protection offered by omega-3-rich pork lard against hyperlipidemia indicated that pork lard could be used as alternative cooking oil for health-conscious individuals. It could also be introduced as a functional ingredient for patients with hyperlipidemia.2022-01-01T00:00:00Z20222025-04-04T11:48:20.825Z2025-04-04T11:48:20.825ZS-EPMC97083463645820510.1155/2022/9269968