{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["21"],"submitter":["Murakami S"],"pubmed_abstract":["Background Can Background be changed from bold to normal text? Chemically-induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine. We aimed to clarify the efficacy of CLiPs for ameliorating fibrosis in a diet-induced nonalcoholic steatohepatitis rat model, since nonalcoholic fatty liver disease is currently recognized as the most common form of chronic liver disease in developed countries. Methods: Primary mature hepatocytes were isolated from 7-week-old male Wistar rats. To establish CLiPs, isolated hepatocytes were cultured in differentiation medium composed of Y-27632, A-83-01, and CHIR99021 (YAC medium). As an animal model that reproduces NASH pathophysiology, 6-week-old severe combined immunodeficient (SCID) mice were carefully selected and prepare"],"journal":["Regenerative therapy"],"pagination":["574-583"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9708542"],"repository":["biostudies-literature"],"pubmed_title":["Transplantation of chemically-induced liver progenitor cells ameliorates hepatic fibrosis in mice with diet-induced nonalcoholic steatohepatitis"],"pmcid":["PMC9708542"],"pubmed_authors":["Soyama A","Miyamoto D","Matsuguma K","Matsushima H","Ochiya T","Maruya Y","Miuma S","Hidaka M","Hara T","Tanaka T","Kanetaka K","Eguchi S","Imamura H","Murakami S","Adachi T"],"additional_accession":[]},"is_claimable":false,"name":"Transplantation of chemically-induced liver progenitor cells ameliorates hepatic fibrosis in mice with diet-induced nonalcoholic steatohepatitis","description":"Background Can Background be changed from bold to normal text? Chemically-induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine. We aimed to clarify the efficacy of CLiPs for ameliorating fibrosis in a diet-induced nonalcoholic steatohepatitis rat model, since nonalcoholic fatty liver disease is currently recognized as the most common form of chronic liver disease in developed countries. Methods: Primary mature hepatocytes were isolated from 7-week-old male Wistar rats. To establish CLiPs, isolated hepatocytes were cultured in differentiation medium composed of Y-27632, A-83-01, and CHIR99021 (YAC medium). As an animal model that reproduces NASH pathophysiology, 6-week-old severe combined immunodeficient (SCID) mice were carefully selected and prepare","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-26T20:45:13.396Z","creation":"2025-04-06T16:31:30.018Z"},"accession":"S-EPMC9708542","cross_references":{}}