{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["102(12)"],"submitter":["Kreutzer L"],"pubmed_abstract":["Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) allows spatial analysis of proteins, metabolites, or small molecules from tissue sections. Here, we present the simultaneous generation and analysis of MALDI-MSI, whole-exome sequencing (WES), and RNA-sequencing data from the same formalin-fixed paraffin-embedded (FFPE) tissue sections. Genomic DNA and total RNA were extracted from (i) untreated, (ii) hematoxylin-eosin (HE) stained, and (iii) MALDI-MSI-analyzed FFPE tissue sections from three head and neck squamous cell carcinomas. MALDI-MSI data were generated by a time-of-flight analyzer prior to preprocessing and visualization. WES data were generated using a low-input protocol followed by detection of single-nucleotide variants (SNVs), tumor mutational bu"],"journal":["Laboratory investigation; a journal of technical methods and pathology"],"pagination":["1400-1405"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9708593"],"repository":["biostudies-literature"],"pubmed_title":["Simultaneous metabolite MALDI-MSI, whole exome and transcriptome analysis from formalin-fixed paraffin-embedded tissue sections."],"pmcid":["PMC9708593"],"pubmed_authors":["Kreutzer L","Riedl T","Baumeister P","Hess J","Zitzelsberger H","Unger K","Belka C","Klauschen F","Walch A","Weber P","Heider T","Heikenwalder M"],"additional_accession":[]},"is_claimable":false,"name":"Simultaneous metabolite MALDI-MSI, whole exome and transcriptome analysis from formalin-fixed paraffin-embedded tissue sections.","description":"Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) allows spatial analysis of proteins, metabolites, or small molecules from tissue sections. Here, we present the simultaneous generation and analysis of MALDI-MSI, whole-exome sequencing (WES), and RNA-sequencing data from the same formalin-fixed paraffin-embedded (FFPE) tissue sections. Genomic DNA and total RNA were extracted from (i) untreated, (ii) hematoxylin-eosin (HE) stained, and (iii) MALDI-MSI-analyzed FFPE tissue sections from three head and neck squamous cell carcinomas. MALDI-MSI data were generated by a time-of-flight analyzer prior to preprocessing and visualization. WES data were generated using a low-input protocol followed by detection of single-nucleotide variants (SNVs), tumor mutational bu","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-19T18:55:34.556Z","creation":"2025-04-19T18:55:34.556Z"},"accession":"S-EPMC9708593","cross_references":{"pubmed":["36045222"],"doi":["10.1038/s41374-022-00829-0"]}}