biostudies-literatureUnknown13Park JPlant bacterial disease is a complex outcome achieved through a combination of virulence factors that are activated during infection. However, the common virulence factors across diverse plant pathogens are largely uncharacterized. Here, we established a pan-genome shared across the following plant pathogens: <i>Burkholderia glumae</i>, <i>Ralstonia solanacearum</i>, and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>. By overlaying <i>in planta</i> transcriptomes onto the pan-genome, we investigated the expression profiles of common genes during infection. We found over 70% of identical patterns for genes commonly expressed by the pathogens in different plant hosts or infection sites. Co-expression patterns revealed the activation of a signal transduction cascade to recognize and respond to eFrontiers in plant science1030720https://www.ebi.ac.uk/biostudies/studies/S-EPMC9709210biostudies-literatureGenome-guided comparative <i>in planta</i> transcriptome analyses for identifying cross-species common virulence factors in bacterial phytopathogens.PMC9709210Park JLee SYPark DSJung HMannaa MLee SWHan GLee HHSeo YSKim NfalseGenome-guided comparative <i>in planta</i> transcriptome analyses for identifying cross-species common virulence factors in bacterial phytopathogens.Plant bacterial disease is a complex outcome achieved through a combination of virulence factors that are activated during infection. However, the common virulence factors across diverse plant pathogens are largely uncharacterized. Here, we established a pan-genome shared across the following plant pathogens: <i>Burkholderia glumae</i>, <i>Ralstonia solanacearum</i>, and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>. By overlaying <i>in planta</i> transcriptomes onto the pan-genome, we investigated the expression profiles of common genes during infection. We found over 70% of identical patterns for genes commonly expressed by the pathogens in different plant hosts or infection sites. Co-expression patterns revealed the activation of a signal transduction cascade to recognize and respond to e2022-01-01T00:00:00Z20222026-05-29T18:52:36.72Z2024-11-06T14:26:24.513ZS-EPMC97092103646624910.3389/fpls.2022.1030720