biostudies-literatureSharma RNational Natural Science Foundation of China1063118https://www.ebi.ac.uk/biostudies/studies/S-EPMC9709420biostudies-literatureUnknown9<i>Trikatu Churna</i> (<i>TC</i>) comprising <i>Zingiber officinale</i> rhizome, <i>Piper longum</i>, and <i>Piper nigrum</i> fruit, is effective in treating liver diseases and has high nutraceutical values. However, the efficacy of <i>TC</i> in treating alcoholic liver disease (ALD) and its mechanism remain largely unknown. This study evaluated the hepatoprotective effects of different doses of <i>TC</i> as well as to identify the bioactive components and determine their mechanism of action against ethanol-induced ALD. A compound-target network analysis model of <i>TC</i> was established to identify its potential bioactive compounds and pathways that might regulate its hepatoprotective effects. Further, <i>in-vivo</i> studies were performed to validate the potential of <i>TC</i> (200 mg/kFrontiers in nutritionDeciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and <i>in vivo</i> validation.PMC970942032070671Jadhav MSharma RGundamaraju RAlAsmari AFAli NRauf AShen BChoudhary NKumar ASingla RKfalseDeciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and <i>in vivo</i> validation.<i>Trikatu Churna</i> (<i>TC</i>) comprising <i>Zingiber officinale</i> rhizome, <i>Piper longum</i>, and <i>Piper nigrum</i> fruit, is effective in treating liver diseases and has high nutraceutical values. However, the efficacy of <i>TC</i> in treating alcoholic liver disease (ALD) and its mechanism remain largely unknown. This study evaluated the hepatoprotective effects of different doses of <i>TC</i> as well as to identify the bioactive components and determine their mechanism of action against ethanol-induced ALD. A compound-target network analysis model of <i>TC</i> was established to identify its potential bioactive compounds and pathways that might regulate its hepatoprotective effects. Further, <i>in-vivo</i> studies were performed to validate the potential of <i>TC</i> (200 mg/k2022-01-01T00:00:00Z20222026-04-08T11:37:31.692Z2024-11-20T21:36:18.708ZS-EPMC97094203646641710.3389/fnut.2022.1063118