biostudies-literatureUnknown23(1)Tippani M<h4>Background</h4>Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced pluripotent stem cells (hiPSCs), are lacking. Existing methods lack scalability to accommodate high-throughput comparisons between multiple lines, across developmental timepoints, or across pharmacological manipulations.<h4>Results</h4>To address this need we developed CaPTure, a scalable, automated Ca²⁺ imaging analysis pipeline ( https://github.com/LieberInstitute/CaPTure ). CaPTuredetects neurons, classifies and quantifies spontaneous activity, quantifies synchrony metrics, and generates cell- and network-specific metricBMC neuroscience71https://www.ebi.ac.uk/biostudies/studies/S-EPMC9710137biostudies-literatureCaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data.PMC9710137Pattie EAMartinowich KPage SCNguyen CVMaher BJTippani MDavis BASripathy SRJaffe AEWang YfalseCaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data.<h4>Background</h4>Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced pluripotent stem cells (hiPSCs), are lacking. Existing methods lack scalability to accommodate high-throughput comparisons between multiple lines, across developmental timepoints, or across pharmacological manipulations.<h4>Results</h4>To address this need we developed CaPTure, a scalable, automated Ca²⁺ imaging analysis pipeline ( https://github.com/LieberInstitute/CaPTure ). CaPTuredetects neurons, classifies and quantifies spontaneous activity, quantifies synchrony metrics, and generates cell- and network-specific metric2022-01-01T00:00:00Z2022 Nov2025-04-18T19:19:47.947Z2025-04-07T07:00:33.342ZS-EPMC97101373645108910.1186/s12868-022-00751-7