biostudies-literatureLiu ZNIGMS NIH HHS332-339https://www.ebi.ac.uk/biostudies/studies/S-EPMC9710542biostudies-literatureUnknown605(7909)Stomata exert considerable effects on global carbon and water cycles by mediating gas exchange and water vapour^1,2. Stomatal closure prevents water loss in response to dehydration and limits pathogen entry^3,4. However, prolonged stomatal closure reduces photosynthesis and transpiration and creates aqueous apoplasts that promote colonization by pathogens. How plants dynamically regulate stomatal reopening in a changing climate is unclear. Here we show that the secreted peptides SMALL PHYTOCYTOKINES REGULATING DEFENSE AND WATER LOSS (SCREWs) and the cognate receptor kinase PLANT SCREW UNRESPONSIVE RECEPTOR (NUT) counter-regulate phytohormone abscisic acid (ABA)- and microbe-associated molecular pattern (MAMP)-induced stomatal closure. SCREWs sensed by NUT function as imNaturePhytocytokine signalling reopens stomata in plant immunity and water loss.PMC9710542R01 GM092893R01 GM097247R35 GM144275Liu JLei JRodrigues OShan LZhu-Salzman KOrtiz-Morea FAHou SWang HHe SYLuo DHe PZhang WLiu ZWang PMunemasa SNomura KYin CWang XfalsePhytocytokine signalling reopens stomata in plant immunity and water loss.Stomata exert considerable effects on global carbon and water cycles by mediating gas exchange and water vapour^1,2. Stomatal closure prevents water loss in response to dehydration and limits pathogen entry^3,4. However, prolonged stomatal closure reduces photosynthesis and transpiration and creates aqueous apoplasts that promote colonization by pathogens. How plants dynamically regulate stomatal reopening in a changing climate is unclear. Here we show that the secreted peptides SMALL PHYTOCYTOKINES REGULATING DEFENSE AND WATER LOSS (SCREWs) and the cognate receptor kinase PLANT SCREW UNRESPONSIVE RECEPTOR (NUT) counter-regulate phytohormone abscisic acid (ABA)- and microbe-associated molecular pattern (MAMP)-induced stomatal closure. SCREWs sensed by NUT function as im2022-01-01T00:00:00Z2022 May2025-04-05T10:17:37.923Z2025-02-19T04:19:56.706ZS-EPMC97105423550865910.1038/s41586-022-04684-3