{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stankovic D"],"funding":["Deutsche Forschungsgemeinschaft","NIH HHS"],"pagination":["e202201801"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9711858"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(2)"],"pubmed_abstract":["Post-transcriptional gene silencing using double-stranded RNA has revolutionized the field of functional genetics, allowing fast and easy disruption of gene function in various organisms. In <i>Drosophila</i>, many transgenic RNAi lines have been generated in large-scale efforts, including the <i>Drosophila</i> Transgenic RNAi Project (TRiP), to facilitate in vivo knockdown of virtually any <i>Drosophila</i> gene with spatial and temporal resolution. The available transgenic RNAi lines represent a fundamental resource for the fly community, providing an unprecedented opportunity to address a vast range of biological questions relevant to basic and biomedical research fields. However, caution should be applied regarding the efficiency and specificity of the RNAi approach. Here, we demonstra"],"journal":["Life science alliance"],"pubmed_title":["<i>Drosophila</i> pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes."],"pmcid":["PMC9711858"],"funding_grant_id":["Germany&apos;s Excellence Strategy – CECAD, EXC 2030 – 390661388","P40 OD010949"],"pubmed_authors":["Csordas G","Stankovic D","Uhlirova M"],"additional_accession":[]},"is_claimable":false,"name":"<i>Drosophila</i> pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes.","description":"Post-transcriptional gene silencing using double-stranded RNA has revolutionized the field of functional genetics, allowing fast and easy disruption of gene function in various organisms. In <i>Drosophila</i>, many transgenic RNAi lines have been generated in large-scale efforts, including the <i>Drosophila</i> Transgenic RNAi Project (TRiP), to facilitate in vivo knockdown of virtually any <i>Drosophila</i> gene with spatial and temporal resolution. The available transgenic RNAi lines represent a fundamental resource for the fly community, providing an unprecedented opportunity to address a vast range of biological questions relevant to basic and biomedical research fields. However, caution should be applied regarding the efficiency and specificity of the RNAi approach. Here, we demonstra","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-05-29T04:34:04.946Z","creation":"2025-04-04T19:37:59.641Z"},"accession":"S-EPMC9711858","cross_references":{"pubmed":["36446522"],"doi":["10.26508/lsa.202201801"]}}