{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Scally SW"],"funding":["Department of Health | National Health and Medical Research Council","Wellcome Trust"],"pagination":["2039-2053"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712106"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(12)"],"pubmed_abstract":["The most severe form of malaria is caused by Plasmodium falciparum. These parasites invade human erythrocytes, and an essential step in this process involves the ligand PfRh5, which forms a complex with cysteine-rich protective antigen (CyRPA) and PfRh5-interacting protein (PfRipr) (RCR complex) and binds basigin on the host cell. We identified a heteromeric disulfide-linked complex consisting of P. falciparum Plasmodium thrombospondin-related apical merozoite protein (PfPTRAMP) and P. falciparum cysteine-rich small secreted protein (PfCSS) and have shown that it binds RCR to form a pentameric complex, PCRCR. Using P. falciparum lines with conditional knockouts, invasion inhibitory nanobodies to both PfPTRAMP and PfCSS, and lattice light-sheet microscopy, we show that they are essential fo"],"journal":["Nature microbiology"],"pubmed_title":["PCRCR complex is essential for invasion of human erythrocytes by Plasmodium falciparum."],"pmcid":["PMC9712106"],"funding_grant_id":["206515/Z/17/Z","1173049","637406"],"pubmed_authors":["Rogers KL","Evelyn C","Scally SW","Geoghegan ND","Adair A","Cowman AF","Pasternak M","Healer J","Triglia T","Seager BA","Dagley LF","Lim PS","Tham WH"],"additional_accession":[]},"is_claimable":false,"name":"PCRCR complex is essential for invasion of human erythrocytes by Plasmodium falciparum.","description":"The most severe form of malaria is caused by Plasmodium falciparum. These parasites invade human erythrocytes, and an essential step in this process involves the ligand PfRh5, which forms a complex with cysteine-rich protective antigen (CyRPA) and PfRh5-interacting protein (PfRipr) (RCR complex) and binds basigin on the host cell. We identified a heteromeric disulfide-linked complex consisting of P. falciparum Plasmodium thrombospondin-related apical merozoite protein (PfPTRAMP) and P. falciparum cysteine-rich small secreted protein (PfCSS) and have shown that it binds RCR to form a pentameric complex, PCRCR. Using P. falciparum lines with conditional knockouts, invasion inhibitory nanobodies to both PfPTRAMP and PfCSS, and lattice light-sheet microscopy, we show that they are essential fo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-05-28T21:28:52.661Z","creation":"2025-04-19T22:48:46.968Z"},"accession":"S-EPMC9712106","cross_references":{"pubmed":["36396942"],"doi":["10.1038/s41564-022-01261-2"]}}