biostudies-literatureRasheduzzaman MChamindie PunyadeeraMohammad RasheduzzamanDaniel KolarichGriffith University8401-8411https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712327biostudies-literatureUnknown414(29-30)Glycosylation is the most common post-translational modification of proteins, and glycosylation changes at cell surfaces are frequently associated with malignant epithelia including head and neck squamous cell carcinoma (HNSCC). In HNSCC, 5-year survival remains poor, averaging around 50% globally: this is partly related to late diagnosis. Specific protein glycosylation signatures on malignant keratinocytes have promise as diagnostic and prognostic biomarkers and as therapeutic targets. Nevertheless, HNSCC-specific glycome is to date largely unknown. Herein, we tested six established HNSCC cell lines to capture the qualitative and semi-quantitative N-glycome using porous graphitized carbon liquid chromatography coupled to electrospray ionisation tandem mass spectrometry. Oligomannose-type Analytical and bioanalytical chemistryHead and neck cancer N-glycome traits are cell line and HPV status-dependent.PMC9712327Medical Research Future Fund (MRFF)Cancer Australia (APP1145657)Australian Government Research Training ProgramMedical Research CouncilAustralian Government grant by project CE140100003Australian Research Council Future FellowshipNational HealthDolcetti RMurugan AVMKolarich DKenny LZhang XOliveira TJohnson NWPunyadeera CRasheduzzaman MfalseHead and neck cancer N-glycome traits are cell line and HPV status-dependent.Glycosylation is the most common post-translational modification of proteins, and glycosylation changes at cell surfaces are frequently associated with malignant epithelia including head and neck squamous cell carcinoma (HNSCC). In HNSCC, 5-year survival remains poor, averaging around 50% globally: this is partly related to late diagnosis. Specific protein glycosylation signatures on malignant keratinocytes have promise as diagnostic and prognostic biomarkers and as therapeutic targets. Nevertheless, HNSCC-specific glycome is to date largely unknown. Herein, we tested six established HNSCC cell lines to capture the qualitative and semi-quantitative N-glycome using porous graphitized carbon liquid chromatography coupled to electrospray ionisation tandem mass spectrometry. Oligomannose-type 2022-01-01T00:00:00Z2022 Dec2026-05-05T02:50:37.728Z2025-04-06T04:50:13.582ZS-EPMC97123273628910310.1007/s00216-022-04376-x