biostudies-literatureUnknown13Pang YBacterial extracellular membrane vesicles (MV) are potent mediators of microbe-host signals, and they are not only important in host-pathogen interactions but also for the interactions between mutualistic bacteria and their hosts. Studies of MV derived from probiotics could enhance the understanding of these universal signal entities, and here we have studied MV derived from <i>Limosilactobacillus reuteri</i> DSM 17938 and BG-R46. The production of MV increased with cultivation time and after oxygen stress. Mass spectrometry-based proteomics analyses revealed that the MV carried a large number of bacterial cell surface proteins, several predicted to be involved in host-bacteria interactions. A 5'-nucleotidase, which catalyze the conversion of AMP into the signal molecule adenosine, was oneFrontiers in microbiology1032202https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712456biostudies-literatureExtracellular membrane vesicles from <i>Limosilactobacillus reuteri</i> strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1.PMC9712456Sverremark-Ekstrom ERoos SPang YErmann Lundberg LAhl DJonsson HMata Forsberg MPallin AKarlsson RBysell HfalseExtracellular membrane vesicles from <i>Limosilactobacillus reuteri</i> strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1.Bacterial extracellular membrane vesicles (MV) are potent mediators of microbe-host signals, and they are not only important in host-pathogen interactions but also for the interactions between mutualistic bacteria and their hosts. Studies of MV derived from probiotics could enhance the understanding of these universal signal entities, and here we have studied MV derived from <i>Limosilactobacillus reuteri</i> DSM 17938 and BG-R46. The production of MV increased with cultivation time and after oxygen stress. Mass spectrometry-based proteomics analyses revealed that the MV carried a large number of bacterial cell surface proteins, several predicted to be involved in host-bacteria interactions. A 5'-nucleotidase, which catalyze the conversion of AMP into the signal molecule adenosine, was one2022-01-01T00:00:00Z20222026-05-27T22:55:49.501Z2025-04-06T12:12:15.938ZS-EPMC97124563646667110.3389/fmicb.2022.1032202