{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hahn KR"],"funding":["Ministry of Science, ICT and Future Planning"],"pagination":["20659"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712579"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Carboxyl terminus of Hsc70-interacting protein (CHIP) is highly conserved and is linked to the connection between molecular chaperones and proteasomes to degrade chaperone-bound proteins. In this study, we synthesized the transactivator of transcription (Tat)-CHIP fusion protein for effective delivery into the brain and examined the effects of CHIP against oxidative stress in HT22 cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment and ischemic damage in gerbils by 5 min of occlusion of both common carotid arteries, to elucidate the possibility of using Tat-CHIP as a therapeutic agent against ischemic damage. Tat-CHIP was effectively delivered to HT22 hippocampal cells in a concentration- and time-dependent manner, and protein degradation was confirmed in HT22 cells. "],"journal":["Scientific reports"],"pubmed_title":["CHIP ameliorates neuronal damage in H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HT22 cells and gerbil ischemia."],"pmcid":["PMC9712579"],"funding_grant_id":["NRF-2021R1F1A1048079","NRF-2022R1A2B5B01002280"],"pubmed_authors":["Kim DW","Kwon HJ","Yoon YS","Hahn KR","Hwang IK"],"additional_accession":[]},"is_claimable":false,"name":"CHIP ameliorates neuronal damage in H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HT22 cells and gerbil ischemia.","description":"Carboxyl terminus of Hsc70-interacting protein (CHIP) is highly conserved and is linked to the connection between molecular chaperones and proteasomes to degrade chaperone-bound proteins. In this study, we synthesized the transactivator of transcription (Tat)-CHIP fusion protein for effective delivery into the brain and examined the effects of CHIP against oxidative stress in HT22 cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment and ischemic damage in gerbils by 5 min of occlusion of both common carotid arteries, to elucidate the possibility of using Tat-CHIP as a therapeutic agent against ischemic damage. Tat-CHIP was effectively delivered to HT22 hippocampal cells in a concentration- and time-dependent manner, and protein degradation was confirmed in HT22 cells. ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-27T11:53:52.799Z","creation":"2024-11-21T00:16:00.677Z"},"accession":"S-EPMC9712579","cross_references":{"pubmed":["36450819"],"doi":["10.1038/s41598-022-22766-0"]}}