biostudies-literatureHahn KRMinistry of Science, ICT and Future Planning20659https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712579biostudies-literatureUnknown12(1)Carboxyl terminus of Hsc70-interacting protein (CHIP) is highly conserved and is linked to the connection between molecular chaperones and proteasomes to degrade chaperone-bound proteins. In this study, we synthesized the transactivator of transcription (Tat)-CHIP fusion protein for effective delivery into the brain and examined the effects of CHIP against oxidative stress in HT22 cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment and ischemic damage in gerbils by 5 min of occlusion of both common carotid arteries, to elucidate the possibility of using Tat-CHIP as a therapeutic agent against ischemic damage. Tat-CHIP was effectively delivered to HT22 hippocampal cells in a concentration- and time-dependent manner, and protein degradation was confirmed in HT22 cells. Scientific reportsCHIP ameliorates neuronal damage in H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HT22 cells and gerbil ischemia.PMC9712579NRF-2021R1F1A1048079NRF-2022R1A2B5B01002280Kim DWKwon HJYoon YSHahn KRHwang IKfalseCHIP ameliorates neuronal damage in H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HT22 cells and gerbil ischemia.Carboxyl terminus of Hsc70-interacting protein (CHIP) is highly conserved and is linked to the connection between molecular chaperones and proteasomes to degrade chaperone-bound proteins. In this study, we synthesized the transactivator of transcription (Tat)-CHIP fusion protein for effective delivery into the brain and examined the effects of CHIP against oxidative stress in HT22 cells induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment and ischemic damage in gerbils by 5 min of occlusion of both common carotid arteries, to elucidate the possibility of using Tat-CHIP as a therapeutic agent against ischemic damage. Tat-CHIP was effectively delivered to HT22 hippocampal cells in a concentration- and time-dependent manner, and protein degradation was confirmed in HT22 cells. 2022-01-01T00:00:00Z2022 Nov2026-05-27T11:53:52.799Z2024-11-21T00:16:00.677ZS-EPMC97125793645081910.1038/s41598-022-22766-0