biostudies-literatureMa ZGouvernement du Canada | Natural Sciences and Engineering Research Council of CanadaAlberta Innovates | Alberta Innovates - Health SolutionsGouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada)Alberta Innovates | Alberta Innovates - Health Solutions (AIHS)Women and Children's Health Research Institute (Women & Children's Health Research Institute)Canada Foundation for Innovation (Fondation canadienne pour l'innovation)Alberta Cancer FoundationCanada Foundation for InnovationWomen and Children's Health Research InstituteGouvernement du Canada | Canadian Institutes of Health ResearchGouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada)736https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712603biostudies-literatureUnknown9(1)Osteoarthritis (OA) primarily affects mechanical load-bearing joints, with the knee being the most common. The prevalence, burden and severity of knee osteoarthritis (KOA) are disproportionately higher in females, but hormonal differences alone do not explain the disproportionate incidence of KOA in females. Mechanical unloading by spaceflight microgravity has been implicated in OA development in cartilaginous tissues. However, the mechanisms and sex-dependent differences in OA-like development are not well explored. In this study, engineered meniscus constructs were generated from healthy human meniscus fibrochondrocytes (MFC) seeded onto type I collagen scaffolds and cultured under normal gravity and simulated microgravity conditions. We report the whole-genome sequences of constructs frScientific dataMechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity: A Transcriptomic Study.PMC9712603WCHRI Summer StudentshipNSERC RGPIN-2018-06290 AdesidaACF-MIBRP 27128 AdesidaCIHR MOP 125921 AdesidaUOFAB WCHRIIG 3126NSERC RTI-2019-00310 AdesidaSummer StudentshipCFI 33786Undergraduate Student Research AwardsSommerfeldt MAdesida ABLi DXWestover LGraf DMa ZMulet-Sierra AChee RKWKunze MfalseMechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity: A Transcriptomic Study.Osteoarthritis (OA) primarily affects mechanical load-bearing joints, with the knee being the most common. The prevalence, burden and severity of knee osteoarthritis (KOA) are disproportionately higher in females, but hormonal differences alone do not explain the disproportionate incidence of KOA in females. Mechanical unloading by spaceflight microgravity has been implicated in OA development in cartilaginous tissues. However, the mechanisms and sex-dependent differences in OA-like development are not well explored. In this study, engineered meniscus constructs were generated from healthy human meniscus fibrochondrocytes (MFC) seeded onto type I collagen scaffolds and cultured under normal gravity and simulated microgravity conditions. We report the whole-genome sequences of constructs fr2022-01-01T00:00:00Z2022 Nov2026-07-14T15:08:49.911Z2025-04-19T22:47:48.556ZS-EPMC97126033645078510.1038/s41597-022-01837-x