biostudies-literatureShen WNatural Science Foundation of Zhejiang ProvinceNational Natural Science Foundation of China105644https://www.ebi.ac.uk/biostudies/studies/S-EPMC9712682biostudies-literatureUnknown25(12)Linezolid resistance has represented a global concern with its wide dissemination among nosocomial pathogens in recent years. One hundred and two linezolid-resistant <i>Staphylococcus capitis</i> (LRSC) were constantly isolated from 2011 to 2021, which demonstrated single clonal dissemination in a Chinese tertiary hospital. A structurally similar <i>cfr</i>-carrying plasmid was identified among 90 isolates. A chromosomal <i>cfr</i> was located beside a Tn<i>4001</i>-like transposon and IS<i>Enfa4</i> in one strain (LR95). The loss of <i>cfr</i>-carrying plasmid was observed in 11 isolates and the <i>in vitro</i> passage experiments. Conjugation experiments demonstrated the horizontal transferability of the <i>cfr</i>-carrying plasmid into <i>Staphylococcus aureus</i> RN4220. Both <i>cfr</i>-positive LRSC and <i>S. aureus</i> showed no significant differences in growth rates, while only the former displayed competition defect, suggesting this plasmid imposed a certain fitness cost on LRSC. Hence, ongoing measurements are supposed to be adopted to control the spread of these antimicrobial-resistant bacteria.iScienceAn 11-year linezolid-resistant <i>Staphylococcus capitis</i> clone dissemination with a similar <i>cfr</i>-carrying plasmid in China.PMC9712682LY22H20000181971988Chen JCai JZhang RShen WfalseAn 11-year linezolid-resistant <i>Staphylococcus capitis</i> clone dissemination with a similar <i>cfr</i>-carrying plasmid in China.Linezolid resistance has represented a global concern with its wide dissemination among nosocomial pathogens in recent years. One hundred and two linezolid-resistant <i>Staphylococcus capitis</i> (LRSC) were constantly isolated from 2011 to 2021, which demonstrated single clonal dissemination in a Chinese tertiary hospital. A structurally similar <i>cfr</i>-carrying plasmid was identified among 90 isolates. A chromosomal <i>cfr</i> was located beside a Tn<i>4001</i>-like transposon and IS<i>Enfa4</i> in one strain (LR95). The loss of <i>cfr</i>-carrying plasmid was observed in 11 isolates and the <i>in vitro</i> passage experiments. Conjugation experiments demonstrated the horizontal transferability of the <i>cfr</i>-carrying plasmid into <i>Staphylococcus aureus</i> RN4220. Both <i>cfr</i>-positive LRSC and <i>S. aureus</i> showed no significant differences in growth rates, while only the former displayed competition defect, suggesting this plasmid imposed a certain fitness cost on LRSC. Hence, ongoing measurements are supposed to be adopted to control the spread of these antimicrobial-resistant bacteria.2022-01-01T00:00:00Z2022 Dec2026-06-21T03:16:42.022Z2025-02-19T04:14:58.335ZS-EPMC97126823646511910.1016/j.isci.2022.105644