{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dai Z"],"funding":["Tianjin Medical University","Ministry of Human Resources and Social Security","National Natural Science Foundation of China","Natural Science Foundation of Heilongjiang Province"],"pagination":["105642"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9713377"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(12)"],"pubmed_abstract":["Cerebral cavernous malformation (CCM) is caused by loss-of-function mutations in <i>CCM1</i>, <i>CCM2</i>, or <i>CCM3</i> genes of endothelial cells. It is characterized by pericyte deficiency. However, the role of pericytes in CCMs is not yet clarified. We found pericytes in <i>Cdh5Cre</i> <sup><i>ERT2</i></sup> ;<i>Ccm1</i> <sup><i>fl/fl</i></sup> (<i>Ccm1</i> <sup><i>ECKO</i></sup> ) mice had a high expression of PDGFRβ. The inhibition of pericyte function by CP-673451 aggravated the CCM lesion development. RNA-sequencing analysis revealed the molecular traits of pericytes, such as highly expressed ECM-related genes, especially <i>Fn1</i>. Furthermore, KLF4 coupled with phosphorylated SMAD3 (pSMAD3) promoted the transcription of fibronectin in the pericytes of CCM lesions. RGDS peptide, an inhibitor of fibronectin, decreased the lesion area in the cerebella and retinas of <i>Ccm1</i> <sup><i>ECKO</i></sup> mice. Also, human CCM lesions had abundant fibronectin deposition, and pSMAD3- and KLF4-positive pericytes. These findings indicate that pericytes are essential for CCM lesion development, and fibronectin intervention may provide a novel target for therapeutic intervention in such patients."],"journal":["iScience"],"pubmed_title":["Role of pericytes in the development of cerebral cavernous malformations."],"pmcid":["PMC9713377"],"funding_grant_id":["82071298","81771276","ZD2016015"],"pubmed_authors":["Li G","Li J","Zhang D","Zheng X","Du W","Lu D","Dai Z","Yang X","Xiong Z","Li Y","Wang R","Men C","Shi C","Wu S","Wang Y","Yan H"],"additional_accession":[]},"is_claimable":false,"name":"Role of pericytes in the development of cerebral cavernous malformations.","description":"Cerebral cavernous malformation (CCM) is caused by loss-of-function mutations in <i>CCM1</i>, <i>CCM2</i>, or <i>CCM3</i> genes of endothelial cells. It is characterized by pericyte deficiency. However, the role of pericytes in CCMs is not yet clarified. We found pericytes in <i>Cdh5Cre</i> <sup><i>ERT2</i></sup> ;<i>Ccm1</i> <sup><i>fl/fl</i></sup> (<i>Ccm1</i> <sup><i>ECKO</i></sup> ) mice had a high expression of PDGFRβ. The inhibition of pericyte function by CP-673451 aggravated the CCM lesion development. RNA-sequencing analysis revealed the molecular traits of pericytes, such as highly expressed ECM-related genes, especially <i>Fn1</i>. Furthermore, KLF4 coupled with phosphorylated SMAD3 (pSMAD3) promoted the transcription of fibronectin in the pericytes of CCM lesions. RGDS peptide, an inhibitor of fibronectin, decreased the lesion area in the cerebella and retinas of <i>Ccm1</i> <sup><i>ECKO</i></sup> mice. Also, human CCM lesions had abundant fibronectin deposition, and pSMAD3- and KLF4-positive pericytes. These findings indicate that pericytes are essential for CCM lesion development, and fibronectin intervention may provide a novel target for therapeutic intervention in such patients.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-06-21T03:14:44.343Z","creation":"2025-04-19T22:48:36.498Z"},"accession":"S-EPMC9713377","cross_references":{"pubmed":["36465134"],"doi":["10.1016/j.isci.2022.105642"]}}