{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin N"],"funding":["National Natural Science Foundation of China","Natural Science Foundation of Guangdong Province","Shantou University"],"pagination":["1070968"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9713466"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2022"],"pubmed_abstract":["Ovarian aging is associated with a decrease in fecundity. Increased oxidative stress of granulosa cells (GCs) is an important contributor. We thus asked whether there is an oxidative stress-related gene signature in GCs associated with ovarian aging. Public nonhuman primate (NHP) single-cell transcriptome was processed to identify GC cluster. Then, a GC signature for ovarian aging was established based on six oxidative stress-related differentially expressed genes (<i>MAPK1</i>, <i>STK24</i>, <i>AREG</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i>). Receiver operating characteristic (ROC) analysis confirmed good discriminating capacity in both NHP single-cell and human bulk transcriptome datasets. Gene expression levels were investigated using qPCR in the human ovarian granulosa-like tumor cell line (KGN) and mouse GCs. In an oxidative stress model, KGN cells were treated with menadione (7.5 <i>μ</i>M, 24 h) to induce oxidative stress, after which upregulation of <i>MAPK1</i>, <i>STK24</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i> and downregulation of <i>AREG</i> were observed (<i>p</i> < 0.05). In an aging model, KGN cells were continuously cultured for 3 months, leading to increased expressions of all genes (<i>p</i> < 0.05). In GCs of reproductively aged (8-month-old) Kunming mice, upregulated expression of <i>Mapk1</i>, <i>Stk24</i>, <i>Atg7</i>, and <i>Pon2</i> and downregulated expression of <i>Anxa1</i> and <i>Areg</i> were observed (<i>p</i> < 0.01). We therefore here identify a six-gene GC signature associated with oxidative stress and ovarian aging."],"journal":["Oxidative medicine and cellular longevity"],"pubmed_title":["An Oxidative Stress-Related Gene Signature in Granulosa Cells Is Associated with Ovarian Aging."],"pmcid":["PMC9713466"],"funding_grant_id":["81570567","L11112008","2020A1515010054","81950410640","81870432","81571994"],"pubmed_authors":["Sun P","Plosch T","Lin J","Zhou X","Lin N"],"additional_accession":[]},"is_claimable":false,"name":"An Oxidative Stress-Related Gene Signature in Granulosa Cells Is Associated with Ovarian Aging.","description":"Ovarian aging is associated with a decrease in fecundity. Increased oxidative stress of granulosa cells (GCs) is an important contributor. We thus asked whether there is an oxidative stress-related gene signature in GCs associated with ovarian aging. Public nonhuman primate (NHP) single-cell transcriptome was processed to identify GC cluster. Then, a GC signature for ovarian aging was established based on six oxidative stress-related differentially expressed genes (<i>MAPK1</i>, <i>STK24</i>, <i>AREG</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i>). Receiver operating characteristic (ROC) analysis confirmed good discriminating capacity in both NHP single-cell and human bulk transcriptome datasets. Gene expression levels were investigated using qPCR in the human ovarian granulosa-like tumor cell line (KGN) and mouse GCs. In an oxidative stress model, KGN cells were treated with menadione (7.5 <i>μ</i>M, 24 h) to induce oxidative stress, after which upregulation of <i>MAPK1</i>, <i>STK24</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i> and downregulation of <i>AREG</i> were observed (<i>p</i> < 0.05). In an aging model, KGN cells were continuously cultured for 3 months, leading to increased expressions of all genes (<i>p</i> < 0.05). In GCs of reproductively aged (8-month-old) Kunming mice, upregulated expression of <i>Mapk1</i>, <i>Stk24</i>, <i>Atg7</i>, and <i>Pon2</i> and downregulated expression of <i>Anxa1</i> and <i>Areg</i> were observed (<i>p</i> < 0.01). We therefore here identify a six-gene GC signature associated with oxidative stress and ovarian aging.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-06-20T03:16:08.795Z","creation":"2025-04-05T10:34:12.861Z"},"accession":"S-EPMC9713466","cross_references":{"pubmed":["36466095"],"doi":["10.1155/2022/1070968"]}}