biostudies-literatureLin NNational Natural Science Foundation of ChinaNatural Science Foundation of Guangdong ProvinceShantou University1070968https://www.ebi.ac.uk/biostudies/studies/S-EPMC9713466biostudies-literatureUnknown2022Ovarian aging is associated with a decrease in fecundity. Increased oxidative stress of granulosa cells (GCs) is an important contributor. We thus asked whether there is an oxidative stress-related gene signature in GCs associated with ovarian aging. Public nonhuman primate (NHP) single-cell transcriptome was processed to identify GC cluster. Then, a GC signature for ovarian aging was established based on six oxidative stress-related differentially expressed genes (<i>MAPK1</i>, <i>STK24</i>, <i>AREG</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i>). Receiver operating characteristic (ROC) analysis confirmed good discriminating capacity in both NHP single-cell and human bulk transcriptome datasets. Gene expression levels were investigated using qPCR in the human ovarian granulosa-like tumor cell line (KGN) and mouse GCs. In an oxidative stress model, KGN cells were treated with menadione (7.5 <i>μ</i>M, 24 h) to induce oxidative stress, after which upregulation of <i>MAPK1</i>, <i>STK24</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i> and downregulation of <i>AREG</i> were observed (<i>p</i> < 0.05). In an aging model, KGN cells were continuously cultured for 3 months, leading to increased expressions of all genes (<i>p</i> < 0.05). In GCs of reproductively aged (8-month-old) Kunming mice, upregulated expression of <i>Mapk1</i>, <i>Stk24</i>, <i>Atg7</i>, and <i>Pon2</i> and downregulated expression of <i>Anxa1</i> and <i>Areg</i> were observed (<i>p</i> < 0.01). We therefore here identify a six-gene GC signature associated with oxidative stress and ovarian aging.Oxidative medicine and cellular longevityAn Oxidative Stress-Related Gene Signature in Granulosa Cells Is Associated with Ovarian Aging.PMC971346681570567L111120082020A1515010054819504106408187043281571994Sun PPlosch TLin JZhou XLin NfalseAn Oxidative Stress-Related Gene Signature in Granulosa Cells Is Associated with Ovarian Aging.Ovarian aging is associated with a decrease in fecundity. Increased oxidative stress of granulosa cells (GCs) is an important contributor. We thus asked whether there is an oxidative stress-related gene signature in GCs associated with ovarian aging. Public nonhuman primate (NHP) single-cell transcriptome was processed to identify GC cluster. Then, a GC signature for ovarian aging was established based on six oxidative stress-related differentially expressed genes (<i>MAPK1</i>, <i>STK24</i>, <i>AREG</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i>). Receiver operating characteristic (ROC) analysis confirmed good discriminating capacity in both NHP single-cell and human bulk transcriptome datasets. Gene expression levels were investigated using qPCR in the human ovarian granulosa-like tumor cell line (KGN) and mouse GCs. In an oxidative stress model, KGN cells were treated with menadione (7.5 <i>μ</i>M, 24 h) to induce oxidative stress, after which upregulation of <i>MAPK1</i>, <i>STK24</i>, <i>ATG7</i>, <i>ANXA1</i>, and <i>PON2</i> and downregulation of <i>AREG</i> were observed (<i>p</i> < 0.05). In an aging model, KGN cells were continuously cultured for 3 months, leading to increased expressions of all genes (<i>p</i> < 0.05). In GCs of reproductively aged (8-month-old) Kunming mice, upregulated expression of <i>Mapk1</i>, <i>Stk24</i>, <i>Atg7</i>, and <i>Pon2</i> and downregulated expression of <i>Anxa1</i> and <i>Areg</i> were observed (<i>p</i> < 0.01). We therefore here identify a six-gene GC signature associated with oxidative stress and ovarian aging.2022-01-01T00:00:00Z20222026-06-20T03:16:08.795Z2025-04-05T10:34:12.861ZS-EPMC97134663646609510.1155/2022/1070968