{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Nie L"],"pubmed_abstract":["Bacterial aromatic polyketides are usually biosynthesized by the type II polyketide synthase (PKS-II) system. Advances in deoxyribonucleic acid (DNA) sequencing, informatics, and biotechnologies have broadened opportunities for the discovery of aromatic polyketides. Meanwhile, metagenomics is a biotechnology that has been considered as a promising approach for the discovery of novel natural products from uncultured bacteria. Here, we cloned a type II polyketide biosynthetic gene cluster (BGC) from the soil metagenome, and the heterologous expression of this gene cluster in <i>Streptomyces coelicolor</i> M1146 resulted in the production of three anthraquinones, two of which (coelulatins <b>2</b> and <b>3</b>) had special hydroxymethyl and methyloxymethyl modifications at C2 of the polyketide scaffold. Gene deletion and <i>in vitro</i> biochemical characterization indicated that the HemN-like radical S-adenosyl-L-methionine (SAM) enzyme CoeI exhibits methylation and is involved in C2 modification."],"journal":["Frontiers in microbiology"],"pagination":["1040900"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9714029"],"repository":["biostudies-literature"],"pubmed_title":["Biosynthesis of coelulatin for the methylation of anthraquinone featuring HemN-like radical S-adenosyl-L-methionine enzyme."],"pmcid":["PMC9714029"],"pubmed_authors":["Cao M","Feng Z","Wei T","Lyu Y","Wang S","Nie L"],"additional_accession":[]},"is_claimable":false,"name":"Biosynthesis of coelulatin for the methylation of anthraquinone featuring HemN-like radical S-adenosyl-L-methionine enzyme.","description":"Bacterial aromatic polyketides are usually biosynthesized by the type II polyketide synthase (PKS-II) system. Advances in deoxyribonucleic acid (DNA) sequencing, informatics, and biotechnologies have broadened opportunities for the discovery of aromatic polyketides. Meanwhile, metagenomics is a biotechnology that has been considered as a promising approach for the discovery of novel natural products from uncultured bacteria. Here, we cloned a type II polyketide biosynthetic gene cluster (BGC) from the soil metagenome, and the heterologous expression of this gene cluster in <i>Streptomyces coelicolor</i> M1146 resulted in the production of three anthraquinones, two of which (coelulatins <b>2</b> and <b>3</b>) had special hydroxymethyl and methyloxymethyl modifications at C2 of the polyketide scaffold. Gene deletion and <i>in vitro</i> biochemical characterization indicated that the HemN-like radical S-adenosyl-L-methionine (SAM) enzyme CoeI exhibits methylation and is involved in C2 modification.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-28T21:28:58.084Z","creation":"2025-04-19T22:49:28.322Z"},"accession":"S-EPMC9714029","cross_references":{"pubmed":["36466681"],"doi":["10.3389/fmicb.2022.1040900"]}}