biostudies-literatureFan HHMinistry of Science and Technology, TaiwanTaipei Medical University102https://www.ebi.ac.uk/biostudies/studies/S-EPMC9714038biostudies-literatureUnknown29(1)<h4>Background</h4>yqiC is required for colonizing the Salmonella enterica serovar Typhimurium (S. Typhimurium) in human cells; however, how yqiC regulates nontyphoidal Salmonella (NTS) genes to influence bacteria-host interactions remains unclear.<h4>Methods</h4>The global transcriptomes of S. Typhimurium yqiC-deleted mutant (ΔyqiC) and its wild-type strain SL1344 after 2 h of in vitro infection with Caco-2 cells were obtained through RNA sequencing to conduct comparisons and identify major yqiC-regulated genes, particularly those involved in Salmonella pathogenicity islands (SPIs), ubiquinone and menaquinone biosynthesis, electron transportation chains (ETCs), and carbohydrate/energy metabolism. A Seahorse XFp Analyzer and assays of NADH/NAD⁺ and H₂O₂ werJournal of biomedical scienceEffects of colonization-associated gene yqiC on global transcriptome, cellular respiration, and oxidative stress in Salmonella Typhimurium.PMC9714038MOST105-2314-B-038-037-MY3DP2-108-21121-01-O-03-01DP2-107-21121-O-03MOST108-2314-B-038-098-MY3Fan HHHuang STCheng HYChang PRLin PCHuang CHYang LTFang SBChang WCChang YCfalseEffects of colonization-associated gene yqiC on global transcriptome, cellular respiration, and oxidative stress in Salmonella Typhimurium.<h4>Background</h4>yqiC is required for colonizing the Salmonella enterica serovar Typhimurium (S. Typhimurium) in human cells; however, how yqiC regulates nontyphoidal Salmonella (NTS) genes to influence bacteria-host interactions remains unclear.<h4>Methods</h4>The global transcriptomes of S. Typhimurium yqiC-deleted mutant (ΔyqiC) and its wild-type strain SL1344 after 2 h of in vitro infection with Caco-2 cells were obtained through RNA sequencing to conduct comparisons and identify major yqiC-regulated genes, particularly those involved in Salmonella pathogenicity islands (SPIs), ubiquinone and menaquinone biosynthesis, electron transportation chains (ETCs), and carbohydrate/energy metabolism. A Seahorse XFp Analyzer and assays of NADH/NAD⁺ and H₂O₂ wer2022-01-01T00:00:00Z2022 Dec2025-04-18T16:55:15.819Z2025-04-07T04:23:01.253ZS-EPMC97140383645710110.1186/s12929-022-00885-0