{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lu Y"],"funding":["Beijing Natural Science Foundation"],"pagination":["2154-2165"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9714364"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(12)"],"pubmed_abstract":["Mouse bone marrow mesenchymal stem cells (mBM-MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM-MSCs using three easy-to-perform methods (whole bone marrow-adherent culture, density-gradient centrifugation, and bone digestion), and then compared the morphology, proliferation, differentiation, and paracrine factor profiles of the isolated mBM-MSCs. Of these three isolation methods, the bone digestion method resulted in the highest quantity of mBM-MSCs with high growth potential and moderate differentiation. Conversely, the mBM-MSCs isolated through the whole bone marrow-adherent method exhibited the lowest potency for proliferation and differentiation. The differentially expressed factors between mBM-MSCs were primarily those i"],"journal":["FEBS open bio"],"pubmed_title":["A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy-to-perform approaches."],"pmcid":["PMC9714364"],"funding_grant_id":["517100"],"pubmed_authors":["Zhou L","Lu Y","Shi G","Bai L","Han Y","Wang K","Qin C"],"additional_accession":[]},"is_claimable":false,"name":"A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy-to-perform approaches.","description":"Mouse bone marrow mesenchymal stem cells (mBM-MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM-MSCs using three easy-to-perform methods (whole bone marrow-adherent culture, density-gradient centrifugation, and bone digestion), and then compared the morphology, proliferation, differentiation, and paracrine factor profiles of the isolated mBM-MSCs. Of these three isolation methods, the bone digestion method resulted in the highest quantity of mBM-MSCs with high growth potential and moderate differentiation. Conversely, the mBM-MSCs isolated through the whole bone marrow-adherent method exhibited the lowest potency for proliferation and differentiation. The differentially expressed factors between mBM-MSCs were primarily those i","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-07-14T16:56:59.717Z","creation":"2025-04-07T13:42:32.279Z"},"accession":"S-EPMC9714364","cross_references":{"pubmed":["36153697"],"doi":["10.1002/2211-5463.13493"]}}