biostudies-literatureLu YBeijing Natural Science Foundation2154-2165https://www.ebi.ac.uk/biostudies/studies/S-EPMC9714364biostudies-literatureUnknown12(12)Mouse bone marrow mesenchymal stem cells (mBM-MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM-MSCs using three easy-to-perform methods (whole bone marrow-adherent culture, density-gradient centrifugation, and bone digestion), and then compared the morphology, proliferation, differentiation, and paracrine factor profiles of the isolated mBM-MSCs. Of these three isolation methods, the bone digestion method resulted in the highest quantity of mBM-MSCs with high growth potential and moderate differentiation. Conversely, the mBM-MSCs isolated through the whole bone marrow-adherent method exhibited the lowest potency for proliferation and differentiation. The differentially expressed factors between mBM-MSCs were primarily those iFEBS open bioA comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy-to-perform approaches.PMC9714364517100Zhou LLu YShi GBai LHan YWang KQin CfalseA comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy-to-perform approaches.Mouse bone marrow mesenchymal stem cells (mBM-MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM-MSCs using three easy-to-perform methods (whole bone marrow-adherent culture, density-gradient centrifugation, and bone digestion), and then compared the morphology, proliferation, differentiation, and paracrine factor profiles of the isolated mBM-MSCs. Of these three isolation methods, the bone digestion method resulted in the highest quantity of mBM-MSCs with high growth potential and moderate differentiation. Conversely, the mBM-MSCs isolated through the whole bone marrow-adherent method exhibited the lowest potency for proliferation and differentiation. The differentially expressed factors between mBM-MSCs were primarily those i2022-01-01T00:00:00Z2022 Dec2026-07-14T16:56:59.717Z2025-04-07T13:42:32.279ZS-EPMC97143643615369710.1002/2211-5463.13493