{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Das AS"],"funding":["NHLBI NIH HHS","NCI NIH HHS","National Institutes of Health","Gerber Foundation"],"pagination":["1033695"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9714508"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["The small GTPase family is well-studied in cancer and cellular physiology. With 162 annotated human genes, the family has a broad expression throughout cells of the body. Members of the family have multiple exons that require splicing. Yet, the role of splicing within the family has been underexplored. We have studied the splicing dynamics of small GTPases throughout 41,671 samples by integrating Nanopore and Illumina sequencing techniques. Within this work, we have made several discoveries. 1). Using the GTEx long read data of 92 samples, each small GTPase gene averages two transcripts, with 83 genes (51%) expressing two or more isoforms. 2). Cross-tissue analysis of GTEx from 17,382 samples shows 41 genes (25%) expressing two or more protein-coding isoforms. These include protein-changin"],"journal":["Frontiers in cell and developmental biology"],"pubmed_title":["The complex, dynamic SpliceOme of the small GTPase transcripts altered by technique, sex, genetics, tissue specificity, and RNA base editing."],"pmcid":["PMC9714508"],"funding_grant_id":["R01 HL153165","K00 CA245821","K01ES025435 K00CA245821"],"pubmed_authors":["Vaughan RM","Zieba J","Bupp CP","Williams CL","Das AS","Henderson ML","Koehn O","Prokop JW","Li X","Sherry EC","Rajasekaran S","Chhetri SB","Uhl KL","Nissim S"],"additional_accession":[]},"is_claimable":false,"name":"The complex, dynamic SpliceOme of the small GTPase transcripts altered by technique, sex, genetics, tissue specificity, and RNA base editing.","description":"The small GTPase family is well-studied in cancer and cellular physiology. With 162 annotated human genes, the family has a broad expression throughout cells of the body. Members of the family have multiple exons that require splicing. Yet, the role of splicing within the family has been underexplored. We have studied the splicing dynamics of small GTPases throughout 41,671 samples by integrating Nanopore and Illumina sequencing techniques. Within this work, we have made several discoveries. 1). Using the GTEx long read data of 92 samples, each small GTPase gene averages two transcripts, with 83 genes (51%) expressing two or more isoforms. 2). Cross-tissue analysis of GTEx from 17,382 samples shows 41 genes (25%) expressing two or more protein-coding isoforms. These include protein-changin","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-06-01T01:57:25.195Z","creation":"2024-11-14T17:40:15.976Z"},"accession":"S-EPMC9714508","cross_references":{"pubmed":["36467401"],"doi":["10.3389/fcell.2022.1033695"]}}