{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tu Q"],"funding":["National Natural Science Foundation of China"],"pagination":["e13328"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9715356"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["55(12)"],"pubmed_abstract":["<h4>Background</h4>Mitochondrial DNA (mtDNA) is a potent activator for pro-inflammatory response. Dendritic cells (DCs) are immunosuppressed in sepsis, whether mtDNA mediates immunoparalysis in sepsis remains unknown.<h4>Methods</h4>The mRNAs were assessed by qPCR. Flow cytometry was used to measure the expression of costimulatory molecules and the proliferation of CD4<sup>+</sup> T cells. Western blot and immunofluorescence staining were used to analyse the expression of proteins. Cytokine secretion was detected by ELISA. Histology of lung tissue was used to assess the inflammatory injury.<h4>Results</h4>Lipopolysaccharide-induced endotoxemia increased plasma mtDNA levels and immunoparalysis of spleen DCs, while hydrolysing mtDNA reversed immunoparalysis of spleen DCs in vivo. Moreover, c"],"journal":["Cell proliferation"],"pubmed_title":["Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling."],"pmcid":["PMC9715356"],"funding_grant_id":["81701943","81971813"],"pubmed_authors":["Yao L","Zou Y","Li Y","Guo L","Li J","Zhu J","Liu L","Tu Q","Chen F","Liu C","Yuan Y"],"additional_accession":[]},"is_claimable":false,"name":"Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling.","description":"<h4>Background</h4>Mitochondrial DNA (mtDNA) is a potent activator for pro-inflammatory response. Dendritic cells (DCs) are immunosuppressed in sepsis, whether mtDNA mediates immunoparalysis in sepsis remains unknown.<h4>Methods</h4>The mRNAs were assessed by qPCR. Flow cytometry was used to measure the expression of costimulatory molecules and the proliferation of CD4<sup>+</sup> T cells. Western blot and immunofluorescence staining were used to analyse the expression of proteins. Cytokine secretion was detected by ELISA. Histology of lung tissue was used to assess the inflammatory injury.<h4>Results</h4>Lipopolysaccharide-induced endotoxemia increased plasma mtDNA levels and immunoparalysis of spleen DCs, while hydrolysing mtDNA reversed immunoparalysis of spleen DCs in vivo. Moreover, c","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-25T17:07:34.9Z","creation":"2025-04-06T05:02:14.116Z"},"accession":"S-EPMC9715356","cross_references":{"pubmed":["36106559"],"doi":["10.1111/cpr.13328"]}}