biostudies-literatureTu QNational Natural Science Foundation of Chinae13328https://www.ebi.ac.uk/biostudies/studies/S-EPMC9715356biostudies-literatureUnknown55(12)<h4>Background</h4>Mitochondrial DNA (mtDNA) is a potent activator for pro-inflammatory response. Dendritic cells (DCs) are immunosuppressed in sepsis, whether mtDNA mediates immunoparalysis in sepsis remains unknown.<h4>Methods</h4>The mRNAs were assessed by qPCR. Flow cytometry was used to measure the expression of costimulatory molecules and the proliferation of CD4⁺ T cells. Western blot and immunofluorescence staining were used to analyse the expression of proteins. Cytokine secretion was detected by ELISA. Histology of lung tissue was used to assess the inflammatory injury.<h4>Results</h4>Lipopolysaccharide-induced endotoxemia increased plasma mtDNA levels and immunoparalysis of spleen DCs, while hydrolysing mtDNA reversed immunoparalysis of spleen DCs in vivo. Moreover, cCell proliferationMitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling.PMC97153568170194381971813Yao LZou YLi YGuo LLi JZhu JLiu LTu QChen FLiu CYuan YfalseMitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling.<h4>Background</h4>Mitochondrial DNA (mtDNA) is a potent activator for pro-inflammatory response. Dendritic cells (DCs) are immunosuppressed in sepsis, whether mtDNA mediates immunoparalysis in sepsis remains unknown.<h4>Methods</h4>The mRNAs were assessed by qPCR. Flow cytometry was used to measure the expression of costimulatory molecules and the proliferation of CD4⁺ T cells. Western blot and immunofluorescence staining were used to analyse the expression of proteins. Cytokine secretion was detected by ELISA. Histology of lung tissue was used to assess the inflammatory injury.<h4>Results</h4>Lipopolysaccharide-induced endotoxemia increased plasma mtDNA levels and immunoparalysis of spleen DCs, while hydrolysing mtDNA reversed immunoparalysis of spleen DCs in vivo. Moreover, c2022-01-01T00:00:00Z2022 Dec2025-04-25T17:07:34.9Z2025-04-06T05:02:14.116ZS-EPMC97153563610655910.1111/cpr.13328