biostudies-literatureCao YNational Natural Science Foundation of Chinae13322https://www.ebi.ac.uk/biostudies/studies/S-EPMC9715357biostudies-literatureUnknown55(12)<h4>Objectives</h4>Poor oocyte quality is detrimental to fertilization and embryo development, which causes infertility. Cystathionine β-synthase (CBS) is one of the key enzymes modulating the metabolism of homocysteine (Hcy). Studies have shown that CBS plays an important role in female reproduction. However, the role of CBS in regulating oocyte quality during meiotic maturation still needs further investigation.<h4>Materials and methods</h4>Immunohistochemistry, immunofluorescence, drug treatment, western blot, cRNA construct and in vitro transcription, microinjection of morpholino oligo and cRNA were performed for this study.<h4>Results</h4>We found that CBS was expressed both in human and mouse oocytes of follicles. In mouse oocytes, CBS was distributed in the nucleus at germinal vesicCell proliferationCystathionine β-synthase is required for oocyte quality by ensuring proper meiotic spindle assembly.PMC971535732071134Ji DLiu HCao YTian YZhu XZhen PWang WChai JXue KYan WfalseCystathionine β-synthase is required for oocyte quality by ensuring proper meiotic spindle assembly.<h4>Objectives</h4>Poor oocyte quality is detrimental to fertilization and embryo development, which causes infertility. Cystathionine β-synthase (CBS) is one of the key enzymes modulating the metabolism of homocysteine (Hcy). Studies have shown that CBS plays an important role in female reproduction. However, the role of CBS in regulating oocyte quality during meiotic maturation still needs further investigation.<h4>Materials and methods</h4>Immunohistochemistry, immunofluorescence, drug treatment, western blot, cRNA construct and in vitro transcription, microinjection of morpholino oligo and cRNA were performed for this study.<h4>Results</h4>We found that CBS was expressed both in human and mouse oocytes of follicles. In mouse oocytes, CBS was distributed in the nucleus at germinal vesic2022-01-01T00:00:00Z2022 Dec2025-04-25T17:11:50.809Z2025-04-06T04:51:18.647ZS-EPMC97153573605379710.1111/cpr.13322