{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hernandez-Vicens R"],"funding":["Ariel University","Ariel Scientific Innovations","Israel Cancer Association"],"pagination":["e13348"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9715361"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["55(12)"],"pubmed_abstract":["<h4>Objectives</h4>SETDB1 is a methyltransferase responsible for the methylation of histone H3-lysine-9, which is mainly related to heterochromatin formation. SETDB1 is overexpressed in various cancer types and is associated with an aggressive phenotype. In agreement with its activity, it mainly exhibits a nuclear localization; however, in several cell types a cytoplasmic localization was reported. Here we looked for cytoplasmic functions of SETDB1.<h4>Methods</h4>SETDB1 association with microtubules was detected by immunofluorescence and co-sedimentation. Microtubule dynamics were analysed during recovery from nocodazole treatment and by tracking microtubule plus-ends in live cells. Live cell imaging was used to study mitotic kinetics and protein-protein interaction was identified by co-i"],"journal":["Cell proliferation"],"pubmed_title":["SETDB1 regulates microtubule dynamics."],"pmcid":["PMC9715361"],"funding_grant_id":["202220036"],"pubmed_authors":["Pernicone N","Listovsky T","Singh J","Hernandez-Vicens R","Gerlitz G"],"additional_accession":[]},"is_claimable":false,"name":"SETDB1 regulates microtubule dynamics.","description":"<h4>Objectives</h4>SETDB1 is a methyltransferase responsible for the methylation of histone H3-lysine-9, which is mainly related to heterochromatin formation. SETDB1 is overexpressed in various cancer types and is associated with an aggressive phenotype. In agreement with its activity, it mainly exhibits a nuclear localization; however, in several cell types a cytoplasmic localization was reported. Here we looked for cytoplasmic functions of SETDB1.<h4>Methods</h4>SETDB1 association with microtubules was detected by immunofluorescence and co-sedimentation. Microtubule dynamics were analysed during recovery from nocodazole treatment and by tracking microtubule plus-ends in live cells. Live cell imaging was used to study mitotic kinetics and protein-protein interaction was identified by co-i","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-05-05T18:11:27.308Z","creation":"2026-04-07T21:46:55.263Z"},"accession":"S-EPMC9715361","cross_references":{"pubmed":["36330589"],"doi":["10.1111/cpr.13348"]}}