biostudies-literatureHernandez-Vicens RAriel UniversityAriel Scientific InnovationsIsrael Cancer Associatione13348https://www.ebi.ac.uk/biostudies/studies/S-EPMC9715361biostudies-literatureUnknown55(12)<h4>Objectives</h4>SETDB1 is a methyltransferase responsible for the methylation of histone H3-lysine-9, which is mainly related to heterochromatin formation. SETDB1 is overexpressed in various cancer types and is associated with an aggressive phenotype. In agreement with its activity, it mainly exhibits a nuclear localization; however, in several cell types a cytoplasmic localization was reported. Here we looked for cytoplasmic functions of SETDB1.<h4>Methods</h4>SETDB1 association with microtubules was detected by immunofluorescence and co-sedimentation. Microtubule dynamics were analysed during recovery from nocodazole treatment and by tracking microtubule plus-ends in live cells. Live cell imaging was used to study mitotic kinetics and protein-protein interaction was identified by co-iCell proliferationSETDB1 regulates microtubule dynamics.PMC9715361202220036Pernicone NListovsky TSingh JHernandez-Vicens RGerlitz GfalseSETDB1 regulates microtubule dynamics.<h4>Objectives</h4>SETDB1 is a methyltransferase responsible for the methylation of histone H3-lysine-9, which is mainly related to heterochromatin formation. SETDB1 is overexpressed in various cancer types and is associated with an aggressive phenotype. In agreement with its activity, it mainly exhibits a nuclear localization; however, in several cell types a cytoplasmic localization was reported. Here we looked for cytoplasmic functions of SETDB1.<h4>Methods</h4>SETDB1 association with microtubules was detected by immunofluorescence and co-sedimentation. Microtubule dynamics were analysed during recovery from nocodazole treatment and by tracking microtubule plus-ends in live cells. Live cell imaging was used to study mitotic kinetics and protein-protein interaction was identified by co-i2022-01-01T00:00:00Z2022 Dec2026-05-05T18:11:27.308Z2026-04-07T21:46:55.263ZS-EPMC97153613633058910.1111/cpr.13348