{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rossetta A"],"funding":["European Research Council"],"pagination":["7406"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9715684"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and fluorescence lifetime fluctuation spectroscopy. We expect that our BrightEyes-TTM will support the microscopy community in spreading SP-LSM in many life science laboratories."],"journal":["Nature communications"],"pubmed_title":["The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy."],"pmcid":["PMC9715684"],"funding_grant_id":["818699"],"pubmed_authors":["Fersini F","Rossetta A","Donato M","Lanzano L","Diotalevi F","Perego E","Barberis A","Tortarolo G","Slenders E","Vicidomini G","Koho S","Zappone S","Bruno M","Crepaldi M"],"additional_accession":[]},"is_claimable":false,"name":"The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy.","description":"Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and fluorescence lifetime fluctuation spectroscopy. We expect that our BrightEyes-TTM will support the microscopy community in spreading SP-LSM in many life science laboratories.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-18T16:56:32.014Z","creation":"2025-04-07T04:24:59.699Z"},"accession":"S-EPMC9715684","cross_references":{"pubmed":["36456575"],"doi":["10.1038/s41467-022-35064-0"]}}