biostudies-literatureGhasemi AUniversity of FloridaNIAID NIH HHSNational Institutes of Health1034683https://www.ebi.ac.uk/biostudies/studies/S-EPMC9716130biostudies-literatureUnknown13<i>Helicobacter pylori</i> is a major cause of gastric mucosal inflammation, peptic ulcers, and gastric cancer. Emerging antimicrobial-resistant <i>H. pylori</i> has hampered the effective eradication of frequent chronic infections. Moreover, a safe vaccine is highly demanded due to the absence of effective vaccines against <i>H. pylori</i>. In this study, we employed a new innovative Protective Immunity Enhanced <i>Salmonella</i> Vaccine (PIESV) vector strain to deliver and express multiple <i>H. pylori</i> antigen genes. Immunization of mice with our vaccine delivering the HpaA, Hp-NAP, UreA and UreB antigens, provided sterile protection against <i>H. pylori</i> SS1 infection in 7 out of 10 tested mice. In comparison to the control groups that had received PBS or a PIESV carrying an empty vector, immunized mice exhibited specific and significant cellular recall responses and antigen-specific serum IgG1, IgG2c, total IgG and gastric IgA antibody titers. In conclusion, an improved <i>S.</i> Typhimurium-based live vaccine delivering four antigens shows promise as a safe and effective vaccine against <i>H. pylori</i> infection.Frontiers in immunologyProtective immunity enhanced <i>Salmonella</i> vaccine vectors delivering <i>Helicobacter pylori</i> antigens reduce <i>H. pylori</i> stomach colonization in mice.PMC9716130R01 AI060557P0109858R01 AI60557 , R21 AI126172R21 AI126172Wang SSahay BAbbott JRGhasemi ACurtiss RfalseProtective immunity enhanced <i>Salmonella</i> vaccine vectors delivering <i>Helicobacter pylori</i> antigens reduce <i>H. pylori</i> stomach colonization in mice.<i>Helicobacter pylori</i> is a major cause of gastric mucosal inflammation, peptic ulcers, and gastric cancer. Emerging antimicrobial-resistant <i>H. pylori</i> has hampered the effective eradication of frequent chronic infections. Moreover, a safe vaccine is highly demanded due to the absence of effective vaccines against <i>H. pylori</i>. In this study, we employed a new innovative Protective Immunity Enhanced <i>Salmonella</i> Vaccine (PIESV) vector strain to deliver and express multiple <i>H. pylori</i> antigen genes. Immunization of mice with our vaccine delivering the HpaA, Hp-NAP, UreA and UreB antigens, provided sterile protection against <i>H. pylori</i> SS1 infection in 7 out of 10 tested mice. In comparison to the control groups that had received PBS or a PIESV carrying an empty vector, immunized mice exhibited specific and significant cellular recall responses and antigen-specific serum IgG1, IgG2c, total IgG and gastric IgA antibody titers. In conclusion, an improved <i>S.</i> Typhimurium-based live vaccine delivering four antigens shows promise as a safe and effective vaccine against <i>H. pylori</i> infection.2022-01-01T00:00:00Z20222026-05-07T13:21:47.222Z2025-02-19T04:34:17.566ZS-EPMC97161303646684710.3389/fimmu.2022.1034683