biostudies-literatureUnknown3(4)Rocha B<h4>Objective</h4>The aim of this study was to carry out a targeted phospholipidomic analysis on synovial fluid (SF) from patients with different grades of osteoarthritis (OA) and controls, in order to search for specific phospholipid profiles that may be useful for the deep phenotyping of this disease.<h4>Design</h4>Multiple reaction monitoring-mass spectrometry (MRM/MS) was applied to explore the potential phospholipidomic differences in the SF of knee OA patients (n ​= ​15) (subclassified into early- and late-stage OA) and non-OA controls (n ​= ​4). Multivariate statistical analyses conducted by partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) were performed to identify significantly altered phospholipids in OA, characterize phospholipidomiOsteoarthritis and cartilage open100219https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718215biostudies-literatureTargeted phospholipidomic analysis of synovial fluid as a tool for osteoarthritis deep phenotyping.PMC9718215Amoresano APinto GRuiz-Romero CIlliano ABlanco FJCalamia VRocha BfalseTargeted phospholipidomic analysis of synovial fluid as a tool for osteoarthritis deep phenotyping.<h4>Objective</h4>The aim of this study was to carry out a targeted phospholipidomic analysis on synovial fluid (SF) from patients with different grades of osteoarthritis (OA) and controls, in order to search for specific phospholipid profiles that may be useful for the deep phenotyping of this disease.<h4>Design</h4>Multiple reaction monitoring-mass spectrometry (MRM/MS) was applied to explore the potential phospholipidomic differences in the SF of knee OA patients (n ​= ​15) (subclassified into early- and late-stage OA) and non-OA controls (n ​= ​4). Multivariate statistical analyses conducted by partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) were performed to identify significantly altered phospholipids in OA, characterize phospholipidomi2021-01-01T00:00:00Z2021 Dec2025-04-05T10:33:28.704Z2025-04-05T10:33:28.704ZS-EPMC97182153647475810.1016/j.ocarto.2021.100219